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Center for Cell and Gene Therapy

Houston, Texas

Center for Cell and Gene Therapy
Center for Cell and Gene Therapy
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Ann M. Leen, Ph.D.

Ann M. Leen , Ph.D.Assistant Professor
Department of Pediatrics
Section of Hematology-Oncolpgy
Baylor College of Medicine

E-mail: amleen@txch.org
Phone: 832-824-4690

Education

  • Ph.D., CRUK Institute for Cancer Studies, Birmingham, UK
  • Postdoctoral Fellowship, Baylor College of Medicine

Selected Memberships

  • International Society of Cellular Therapy
  • American Society of Gene Therapy

Research Interests

Dr. Leen's research interests involve using cytotoxic T cells (CTL) for viral and malignant diseases. Dr. Leen is a co-principal investigator on a clinical trial assessing the safety of adoptively transferred donor-derived virus-specific CTL for the prophylaxis and treatment of adenoviral infections post allogeneic stem cell transplant. She is also developing and optimizing methods for generating tumor-specific CTL directed against the cancer testis antigens SSX2 and SSX4, which are expressed in a variety of tumors including Hodgkin Reed Sternberg cells. Therefore, the development of effective methods to reactivate and expand tumor-specific T cells directed against these antigens will lead to the development of immunotherapy protocols for the treatment of patients with relapsed EBV negative Hodgkin's disease and non-Hodgkin Lymphoma.

Selected Publications

  • G Douglas Myers, Catherine M Bollard, Meng-Fen Wu, Heidi Weiss, Cliona M Rooney, Helen E Heslop and Ann M Leen. Reconstitution of Adenovirus Specific Cell Mediated Immunity in Pediatric Patients after Hematopoietic Stem Cell Transplantation. Bone Marrow Transplantation 2007, In press.
  • Foster, A. E., Leen, A. M., Lee, T., Okamura, T., Lu, A., Vera, J., Atkinson, R., Dotti, G., and Rooney, C. M. Autologous designer antigen presenting cells by gene modification of T lymphocyte blasts with IL-7 and IL-12. Journal of Immunotherapy 2007, In press.
  • Leen, A. M., Ratnayake, M., Foster, A. E., Rooney, C. M., and Gottschalk, S. Contact activated Monocytes: efficient antigen presenting cells for the stimulation of antigen-specific T cells. Journal of Immunotherapy. 2007 Jan;30(1):96-107.
  • Leen, A. M., Myers, G. D., Sili, U., Huls, M. H., Weiss, H., Leung, K. S., Carrum, G., Krance, R. A., Molldrem, J. J., Gee, A. P., Brenner, M. K., Heslop, H. E., Rooney, C. M. and Bollard, C. M. Monoculture-derived T lymphocytes specific for multiple viruses expand and produce clinically relevant effects in immunocompromised patients. Nature Medicine. 2006 Oct;12(10):1160-6.
  • Leen, A. M., Rooney, C. M., and Foster, A. E. Improving T cell therapy for cancer. Annual Review of Immunology. 2006 Nov 27; [Epub ahead of print].
  • Leen, A. M., Bollard, C. M., Myers, G. D., and Rooney, C. M. Adenoviral Infections in Hematopoietic Stem Cell Transplantation. Biology of Blood and Marrow Transplantation. Mar;12(3):243-251;2006.
  • Leen, A. M., Myers G. D., Bollard C.M., Huls M.H., Sili U., Gee A.P., Heslop H.E. and Rooney C. M. T Cell Immunotherapy for Adenoviral Infections of Stem Cell transplant Recipients. Annals of the New York Academy of Sciences. Dec;1062:104-15; 2005.
  • Straathof, K., Leen, A. M., Buza, E. L., Taylor, G., Huls, M. H., Heslop, H. E., Rooney, C. M., and Bollard, C. M. Characterization of latent membrane 2-specificity in CTL lines from patients with EBV-positive nasopharyngeal carcinoma and lymphoma. Journal of Immunology. Sept;175(6):4137-47;2005.
  • Long H.M., Haigh T.A., Gudgeon N.H., Leen A.M., Tsang C.W., Brooks J., Landais E., Houssaint E., Lee S.P., Rickinson A.B., and Taylor G.S. CD4+ T-cell responses to Epstein-Barr virus (EBV) latent-cycle antigens and the recognition of EBV-transformed lymphoblastoid cell lines. Journal of Virology. Apr;79(8):4896-907;2005.
  • Leen, A.M. and Rooney, C.M. Adenovirus as an emerging pathogen in immunocompromised patients. British Journal of Haematology 128(2):135-4;2005.
  • Leen, A. M., Sili, U., Vanin, E. F., Jewell, A. M., Xie, W., Vignali, D., Piedra, P. A., Brenner, M. K., Rooney, C. M. Conserved CTL epitopes on the adenovirus hexon protein expand subgroup cross-reactive and group-specific CD8+ T cells. Blood 2004 Oct 15;104:2432-40.
  • Bollard, C. M., Straathof, K. C., Huls, H., Leen, A. M., Lacuesta, K., Davis, A., Gottschalk, S., Brenner, M. K., Heslop, H. E., Rooney, C. M. The generation and characterization of LMP2-specific CTL for use as adoptive transfer from patients with relapsed EBV-positive Hodgkin disease. Journal of Immunotherapy 27(4),317-327;2004.
  • Leen, A. M., Sili, U., Bollard, C.M., and Rooney, C. M. Human Herpesviruses: Biology, Therapy and Immunoprophylaxis. Chapter. Cambridge Press.
  • Bollard, C. M., Kuehnle, I., Leen, A., Heslop, H. E. Adoptive cellular therapy for viral infections. Biology of Blood and Marrow Transplantation : 10,3,143-155;2004.
  • Leen, A. M., Sili, U., Savoldo, B., Jewell, A. M., Piedra, P. A., Brenner, M. K., Rooney, C. M. Fiber-modified Adenoviruses Generate Sub-group Cross-reactive, Adenovirus-specific Cytotoxic T Lymphocytes for Therapeutic Applications. Blood. 2004 Feb 1;103(3):1101-9.
  • Meij, P., Leen, A., Rickinson, A. B., Verkoeijen, S., Vervoort, M. B. H. J., Bloemena, E., Middeldorp, J. M. Identification and prevalence of CD8+ T-cell responses directed against Epstein-Barr virus-encoded latent membrane protein 1 and latent membrane protein 2. International Journal of Cancer: 2002 May 1;99(1):93-9.
  • Leen, A., Meij, P., Redchenko, I., Middeldorp, J., Bloemena, E., Rickinson, A., and Blake, N. Differential immunogenicity of Epstein-Barr virus latent-cycle proteins for human CD4+ T-helper 1 responses. Journal of Virology, Sept. 2001, 8649-8659.

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