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Center for Cell and Gene Therapy

Houston, Texas

Center for Cell and Gene Therapy
Center for Cell and Gene Therapy
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Cell Processing Facility

The CAGT Cell Processing Facility contains 12 ISO 7 clean rooms with access to an additional three suites to extend capacity when needed. Each suite operates at 20-40 air changes per hour with 50% recirculated air and is at positive pressure with respect to the external corridor. All are equipped with mobile cabinetry with stainless steel worksurfaces, one or more 6” biological safety cabinets, tabletop centrifuge, multiple HEPA-filtered incubators, inverted microscope, refrigerator and PC equipped with barcode scanner. Specialized cell separation, counting, washing and cryopreservation equipment is available to staff.

Equipment and critical facility parameters are continuously monitored via a wireless alarm system, with on and offsite surveillance and data collection. Environmental and production monitoring are performed by the Quality Control Laboratory. Materials and equipment used during manufacturing are tracked using a barcoding system which maintains expiration and calibration records. Quality Assurance provides oversight of all manufacturing and facility operations.

The CPF has a Facility Master File with the U.S. Food and Drug Administration and is accredited by the Foundation for the Accreditation of Cellular Therapy. It is a National Heart Lung and Blood Institute somatic cell processing laboratory under the Production Assistance for Cellular Therapy (PACT) contract program. Under this program it manufactures cellular therapy products for investigators without access to a GMP facility.

Researchers working in The Cell Processing FacilityThe staff have experience in the preparation of:

  • Hematopoietic Progenitor Cells
    • Bone Marrow
    • Peripheral Blood Stem Cells
    • Cord Blood
    • CD34/CD133 Enriched Cells
    • Donor Lymphocytes (Native & Transduced)
    • Mesenchymal Stem Cells
  • Marrow-Derived Cells for Regenerative Medicine - Cardiac and Neurological
  • Antigen-Directed T cells (Native & Transduced):
    • Epstein-Barr virus
    • Adenovirus
    • Cytomegalovirus
    • Tumor Associated Antigens
  • Antigen Presenting Cells (APC):
    • Lymphoblastoid Cells
    • Dendritic Cells
    • Monocytes
  • Cells for cardiac and brain injury repair
  • Transduced and transfected cells
  • Tumor vaccines

Products have been prepared for:

  • CAGT protocols at the Methodist and Texas Children's hospitals in Houston
  • The Production Assistance for Cellular Therapy program
  • Biotechnology companies
  • Investigators and clinicians at institutions worldwide

For additional information on the CPF contact Dr. Adrian Gee

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