(Xinyan Lu, M.D., Director)
As part of the mission of the Cytogenetic Core Laboratory, our staff is dedicated to providing a means for investigators of the Dan L. Duncan Cancer Center, Texas Children's Cancer Center and other affiliated TMC investigators the access to an invaluable technical resource which aids in the pathogenetic discoveries in both pediatric and adult neoplasms.
These discoveries may provide insight to the correlation of genetic aberrations with specific diseases and potentially, clinical outcome. Because cytogenetic evaluation of tumors continues to play a key role in the identification of novel gene rearrangements, overexpression, and/or losses of allele heterozygosity through chromosomal loss, it's use as an adjunct technology with other molecular-based testing remains a strong and logical solution for many cancer investigator's questions.
The Cytogenetics Shared Resource Laboratory provides a wide array of state-of-the-art technologies from routine chromosome analysis, to multicolor FISH to characterize complex genotypes, to single and multicolor gene mapping investigation.
Click here to see examples of science supported
• Routine Chromosome Analysis (G-banded Karyotype) on a variety of tissue types
• Fluorescent in situ Hybridization Analysis (FISH) using chromosome painting
• 24-color spectral karyotyping (SKY) - Human or Mouse
• Chromosome comparative genomic hybridization (cCGH) - Human or Mouse
• Gene mapping services including high resolution chromosomal band assignment of single copy genes (Plasmids, PACs, BACs and YACs) by FISH and relative ordering of multiple probes using multicolor FISH.
1. Deletion mapping
2. Identification of translocation junction breakpoints
3. Relative ordering of multiple probes
• Project consultation with Associate Director of Research:
1. Sample types and volumes
2. Project investigator's goals and objectives
3. Timeline for project completion
Charge Back Fees and Forms
Minimum Specimen Requirements
• Cell Lines
T25 or T75 flask with 80~100% confluency in complete media. The lab can split cells and perform metaphase harvest or extract DNA for cCGH.
• Harvested Cell Lines
Contact the laboratory if you plan to submit fixed cells for cytogenetic analysis.
• Purified DNA for cCGH
3-5ug at a concentration of 100ng/ul.
• Whole Bone Marrow or Blood
3.0 cc in Sodium Heparin (green-top) vacutainer.
Transport at Room Temperature or Store at 2-8 C overnight (if necessary).
• Peripheral Blood, High Resolution (non-neoplastic constitutional study)
5.0 cc in Sodium heparin (green-top) vacutainer. Transport at room temperature or store at 2-8 C overnight (if necessary).
• Solid Tissue
~5mm3 tissue biopsy transported in a sealed, STERILE container with RPMI media, Hanks balanced salt solution, or sterile saline. Transport at room temperature or store at 2-8 C overnight (if necessary).
Lab Personnel and Contact Information
Shared Resource Director:
Xinyan Lu, M.D., F.A.C.M.G.
E-mail: firstname.lastname@example.org or email@example.com
Associate Director of Research:
Pulivarthi Rao, Ph.D.
E-mail: firstname.lastname@example.org or email@example.com
Matt R. Folsom, CLDir, CLSp(CG)
E-mail: firstname.lastname@example.org or email@example.com
Yi-Jue Zhao, MD, CLSp(CG)
Lab Phone: 832-824-4768
E-mail:firstname.lastname@example.org or email@example.com
Our Physical Location
Specimen Delivery Address:
Texas Children's Hospital
1102 Bates St., Feigin Center 10th Fl, Ste 1070.20
Houston, TX 77030
Send Mail Correspondence To:
6621 Fannin MC: 3-3320
Houston, TX 77030