Human Tissue Acquisition and Pathology
- Michael M. Ittmann, M.D., Ph.D., Director
- Carolina Gutierrez, M.D., Co-Director
- Daniel Rosen, M.D., Co-Director
The Human Tissue Acquisition and Pathology (HTAP) shared resource offers comprehensive pathology support with the following services:
- Histology and Pathology Services
- Immunohistochemistry (IHC) and TUNEL Assay
- Laser Capture
- Image Analysis
- Pathology consultation
- Tissue microarray Development
- Tissue Acquisition and Distribution
HTAP uses iLabs for all services. For instructions on how to create an account and log-in, visit the iLabs instruction page.
- Tissue/Cell processing and embedding
- Unstained tissue array sectioning
- H&E -staining
- Special stains including: PAS, ORO, Trichrome, VVG for elastin, Giemsa
- Sectioning of thick tissue curls for RNA/DNA isolations
- IHC using core or researcher provided primary antibodies
- Double antibody IHC
- BrdU staining
- Apoptosis/TUNEL assay
Laser capture microdissection with the Arcturus XT instruments is a way for researchers to isolate a particular type of cell or a group of cells with very little contamination. This allows DNA and RNA to be isolated from the cells. The service or amplification of the RNA and/or DNA after isolation is also offered. Tissue samples can be submitted as frozen or paraffin sections.
HTAP can provide state of the art image analysis of standard sections or tissue microarrays using inForm Software. Sections can be stained with standard stains, IHC stains, or immunofluorescence stains. inForm can be trained to find virtually any tissue type, structure, or tissue subtype. Moreover, inForm can provide area object counts or be used to automatically assess IHC staining levels on a cell-by-cell and sub-cellular basis, providing extremely accurate and sensitive quantitation.
- Digital imaging of stained slides
- Digital Scoring of IHC and TUNEL slides
- Image Analysis using Inform and Vectra
One of our experienced pathologists will schedule time to review your slides with you.
HTAP manages an archive that contains formalin fixed paraffin-embedded tissue specimens from a variety of organ/disease types. Approximately 10 to 12 years of clinical, pathological and follow-up data is available for most of these tissue specimens. TMA's can be produced using the core's archived collection and/or researcher provided tissue.
- Identify the cases that meet investigator's project need.
- Investigator's can choose from a library of existing TMAs which include normal and cancer tissues that are site specific or from a multi-tissue array.
- TMA or cell array can be built using specimens provided by the investigator.
- Alternatively, a TMA can be designed to address specific questions in the investigator's proposed research.
- Clinical & pathological data that is associated to each specimen on the array is displayed in the design in an excel format.
- TMA quality control: Tissue/cell type and diagnosis for each specimen on TMA is confirmed by a pathologist.
Supported by the Dan L. Duncan Cancer Center, human tissue banking activities have been expanded to include development of a uniform consent and standardized tissue collection process across all tumor sites at the Michael E. DeBakey Veteran's Affairs Hospital and Ben Taub Hospital.
- Project-based tissue collection
- Tissue acquisition in support of clinical trials and research protocols
- Inventory and data management
Please include the following with your electronic form submission:
- Completed request form
- A summary of your request
- An IRB Protocol or IRB exemption letter
Note: All three of these should be attached as one PDF document and emailed to Michael M. Ittmann, M.D., Ph.D.
Location for Drop Off and Pick up of Specimens (Histology & IHC)
One Baylor Plaza
Margaret Alkek Research Building
Room: R531 C&D
Houston, TX 77030
Location for Drop Off and Pick up of Specimens (LCM & Image Analysis)
One Baylor Plaza
Houston, TX 77030