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Dan L. Duncan Cancer Center

Houston, Texas

BCM has 25 departments and more than 90 research and patient-care centers.
Dan L. Duncan Cancer Center
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MHC Tetramer Production

(X. Lily Wang, M.S., Director)

Services

Provide customized MHC/peptide tetramers for identification of antigen specific T lymphocytes by flow cytometry. Tetramers and/or their derivatives are provided for research purposes only. Their use for commercial purposes is prohibited.

  • Choices of fluorescent labels: R-phycoerythrin (PE) or allophycocyanin (APC)
  • Standard product size: 100μg of MHC/peptide complex protein, which is sufficient for 400 tests (PE labeled) or 250 tests (APC labeled).
  • Other product size: We may split standard vials into smaller aliquots upon request, aliquot size can be as low as 25μg each.
  • Monomers: Monomeric soluble MHC/peptide complex is also available upon request.
  • Clinical Research Grade Reagents: We provide Class I tetramers for certain clinical researches
  • Specialized reagents:

- CD8 binding-deficient MHC I tetramers: Containing mutations in the MHC α3 domain that ablate CD8 binding; Can be used to quantitate and to sort CD8-independent T cells

- Chimeric Tetramer: Containing MHC Human/Mouse chimeric heavy-chain for binding affinity studies

Available MHC Alleles

Please refer to Table 1 for the current available MHC alleles.

If the requested MHC allele is not in the table or if there is other requirements, such as a specific b2-microglobulin is needed to reconstitute class I of an unusual species, the requestor must supply the cDNA. We will perform the subcloning to the appropriate vector for protein expression and additional charges may apply.

All expression plasmids containing MHC alleles will be retained at our facility and will be used for future production and distribution of tetramer reagents.

Pre-Made Common Tetramers

We offer some common class I reagents from a pre-made stock while accepting custom synthesis requests. Please refer to list below:

Human Alleles

MHC Allele
Peptide Sequence
Source of Antigen
HLA-A*0101
VTEHDTLLY
CMV, DNA polymerase processivity factor
HLA-A*0201
MLNIPSINV
CMV, pp65
HLA-A*0201
NLVPMVATV
CMV, pp65
HLA-A*0201
RLLQTGIHV
CMV, pp65
HLA-A*0201
RIFAELEGV
CMV, pp65
HLA-A*0201
LQTGIHVRV
CMV, pp65
HLA-A*2402
FTSQYRIQGKL
CMV, pp65
HLA-A*2402
QYDPVAALF
CMV, pp65
HLA-B*0702
TPRVTGGGAM
CMV, pp65
HLA-B*3501
IPSINVHHY
CMV, pp65
HLA-B*0801
ELRRKMMYM
CMV, IE1
HLA-A*0201
GLCTLVAML
EBV, BMLF1 protein
HLA-A-0201
YLLEILWRL
EBV.LMP1
HLA-A-0201
YLQQNWWTL
EBV.LMP1
HLA-A-0201
TLDYKPLSV
EBV. BMRF1
HLA-A-0201
YVLDHLIVV
EBV. BMLF1
HLA-A-0301
RLRAEAQVK
EBV. EBNA3A
HLA-A-1101
IVTDFSVIK
EBV. EBNA3b
HLA-A-2402
TYGPVFMCL
EBV. LMP2
HLA-A-68012
FTASVSTVV
EBV. LMP2
HLA-B-0702
KILLARLFLY
EBV. LMP2
HLA-B-0801
QAKWRLQTL
EBV. EBNA3A
HLA-B-3501
MGSLEMVPM
EBV. LMP2
HLA- E*01033
SQAPLPCVL
EBV. BZLF1
HLA-A*0201
FLPSDFFPSV
HBV, core
HLA-A*2402
EYLVSFGVW
HBV, core
HLA-A*2402
KYTSFPWLL
HBV, pol
HLA-A*0201
ILKEPVHGV
HIV, pol
HLA-A*0201
SLYNTVATL
HIV, gag
HLA-B*5701
KAFSPEVIPMF
HIV, gag
HLA-A*0201
YMLDLQPET
HPV, E7
HLA-A*0201
LLFGYPVYV
HTLV-1
HLA-A*0201
ELAGIGILTV
Human (melanoma), MART-1
HLA-A*0201
IMDQVPFSV
Human (melanoma), gp100
HLA-A*0201
ITDQVPFSV
Human (melanoma), gp100
HLA-A*0201
KIFGSLAFL
Human/rat/mouse, Her-2/neu
HLA-A*0201
RLLQETELV
Human/rat/mouse, Her-2/neu
HLA-A*0201
VLQELNVTV
Human, proteinase 3
HLA-A*0201
YMDGTMSQV
Human (melanoma), tyrosinase
HLA-A*0201
KTWGQYWQV
Melanoma
HLA-A*0201
GILGFVFTL
Influenza, M1 protein
HLA-B-0702
LPFDRTTVM
Influenza, NP
HLA-A*0201
RMFPNAPYL
WT-1
HLA-A*0201
SLLMWITQC
NY-ESO-1
HLA-A*0201
SLLMWITQC
NY-ESO-1

Mouse Alleles

MHC Allele
Peptide Sequence
Source of Antigen
H-2K(b)
SIINFEKL
Chicken, ovalbumin
H-2D(b)
ASNENMETM
Influenza, NP
H-2D(b)
IPSIQSRGL
Influenza, HA
H-2K(d)
TYQRTRALV
Influenza, NP
H-2D(b)
FQPQNGQFI
LCMV, nucleocapsid
H-2D(b)
KAVYNFATM
LCMV, glycoprotein
H-2D(b)
KAVYNFATC
LCMV, glycoprotein
H-2D(b)
SGVENPGGYCL
LCMV, glycoprotein
H-2K(b)
AVYNFATC
LCMV, glycoprotein
H-2K(b)
AVYNFATCGI
LCMV, glycoprotein
H-2K(b)
ISHNFCNL
LCMV, glycoprotein
H-2K(b)
LEYDFNKL
LCMV, L protein
H-2K(b)
YTVKYPNL
LCMV, nucleocapsid
H-2K(b)
VAYGRQVYL
Listeria monocytogenes
H-2K(d)
GYKDGNEYI
Listeria monocytogenes
H-2K(d)
AMQMLKETI
HIV, gag
H-2D(b)
RAHYNIVTF
HPV16, E7
H-2D(b)
KVPRNQDWL
Human (melanoma), gp100
H-2D(b)
(Abu)(Abu)L(Abu)LTVFL
Moloney murine sarcoma virus (MoMSV)
H-2K(b)
SVYDFFVWL
Mouse, TRP2
H-2D(b)
NAITNAKII
RSV
H-2K(d)
SYIGSINNI
RSV, M2
H-2D(b)
AAVKNWMTQTL
SIV GAG
H-2K(d)
TYDKGILTV
M.tb.HspX
H-2L(d)
MPVGGQSSF
M.tb.Ag85A

Peptide

Requestors incur the cost of peptide that is needed in the tetramer production. You can either provide your own peptide or order from us with an additional cost. If provide your own peptide, please send 10mg of it to us. HPLC chromatograph and mass spectrophotometric analysis for the peptide will also be required.

Charge Back Fees and Forms

· Charge back fees

Please see the charge back fees list for detailed price estimates.

· Request forms

All requestors are required to complete the Tetramer Production Order Form and send order form via e-mail to lxwang@bcm.edu. You will be informed by e-mail when the order is ready to pick up.

Personnel

Xiaochi Lily Wang, M.S.

Director, MHC Tetramer Production Facility

DLDCC Proteomics Shared Resources

Phone: 713-798-3918

E-mail: lxwang@bcm.edu

Physical Location

One Baylor Plaza

DeBakey Building, Rooms M825

Houston, TX 77030

Other Information

MHC Tetramer Order Guidelines

Suggested MHC Tetramer Staining Protocol

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