Super Ovulation and Plugging Schedule
|Thursday||1:00 p.m.||PMSG injection|
|Sunday||Before 10:00 a.m.||Plugs must be checked or they will fall off and not be visible; Plugged females should be placed into a clean cage and must remain in investigator’s animal room until they are brought to the ES Core on Wednesday morning for embryo harvest.|
|Wednesday||Between 9:30 - 10:30 a.m.||Bring plugged females to core.|
- Males must be 7 weeks old and should be separated into individual cages one week prior to time of set-up.
- Females should be between 24-28 days old.
- Hormone injections are administered intraperitoneally using a tuberculine syringe in a total volume of 100ul of 50IU/ml. A total of 5IU/100ul should be administered to each female. Both hormone concentrations should be at a dose of 5IU/100ul. Core will provide hormones to investigators.