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Microscopy

Houston, Texas

A view of cells
Integrated Microscopy Core, Department of Molecular and Cellular Biology
not shown on screen

Applied Precision Deltavision LIVE High Resolution Deconvolution (Image Restoration) Microscope

Applied Precision Deltavision LIVE Deconvolution MicroscopeThe API Deltavision Restoration (Deconvolution) Microscope is an excellent microscope for the acquisition of images on live or fixed cells. The microscope was engineered to provide high contrast and high resolution at low light levels. The environmental chamber provides a temperature controlled, humidified environment with defined CO2 levels. Microscope capabilities include Z-sectioning and 3D reconstruction, time series capture for following cellular events, determining co-localization of multiple proteins and/or dyes and FRAP.

Key features include:

Cameras
  • High Resolution Photometrics Coolsnap Camera
  • High Sensitivity Phtotmetrics HQ2 EMCCD Camera
Acquisition Software
  • SoftWoRx
Filter Sets
  • Live cell filter set - CFP/YFP/RFP or GFP/RFP
  • Standard filter set - DAPI/FITC/TRITC/CY5
  • Alexa filter set - DAPI/FITC/Alexa594/Cy5
  • Brightfield/DIC imaging capabilities
Objectives
  • 40X U Plan-FL N/0.75 NA, air gap
  • 40X Plan-Apo/0.95 NA, air gap with correction collar
  • 40X U Apo/1.35 NA, oil with iris
  • 60X U Plan-Apo/1.20 NA, water with correction collar
  • 60X Plan-Apo/1.4 NA, oil
  • 100X U PlanS-Apo/1.4 NA, oil
Illumination
  • 2 xenon lamps for multiplexing wavelengths
The base microscope is an Olympus IX-71
  • Accepts standard microscope slides, multiwell coverglass chambers, 35mm cell culture dishes and multiwell plates (up to 384 well format)
  • For best results use 1.5 oz (170 micron) coverslips.

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