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Microscopy

Houston, Texas

A view of cells
Integrated Microscopy Core, Department of Molecular and Cellular Biology
not shown on screen

Integrated Microscopy Course

The following courses, offered in 2011, will be offered again beginning in October.

Integrated Microscopy 2011

This class is offered during the 2nd term (elective, 3 credits)
Class begins Oct. 13 and ends Dec. 8, 2011
Class is held every Thursday from 1-5 p.m.

Course Director

Michael Mancini, Ph.D.

Associate Course Director

B.R. Brinkley, Ph.D.

Instructors

  • Michael Mancini, Ph.D.
  • Bill Brinkley, Ph.D.
  • Adam Szafran, M.D., Ph.D.
  • Justin Newberg, Ph.D.

Lectures

Cullen Bldg., Room 130A, every Thursday from 1-2 p.m. (some lectures may run overtime)

Labs

Cullen Bldg., Rooms 120A, 123A and 125A: immediately following lecture, 2-5 p.m. (some labs may run until 6 p.m.)


Class 1 - Oct. 13, 2011

Lecture

Lab

Basic Light Microscopy: Hands on introduction to the light microscope, Kohler illumination and microscope alignment, bright field, DIC


Class 2 - Oct. 18, 2011 (TUESDAY not Thursday): 2:30 - 5:30 p.m.

Lecture

Dr. Broughman - Fundamentals of Light Microscopy II: Absorption, fluorescence, confocal, deconvolution, multi-photon, spectral microscopy, FRAP, FRET, TIRF, live imaging

Lab

Intermediate Light Microscopy: Hands on introduction to confocal and deconvolution microscopy; continued training on standard microscopes


Class 3 - Oct. 27, 2011

Lecture

Dr. Mancini/Dr. Broughman - Advanced Light Microscopy: Introduction to High Throughput Microscopy

Wet-Lab

Cell Labeling/Start of Student Projects: Brightfield; fluorescent labeling of cover slips and multi-well plates with several dyes, antibodies, or expressed fluorescent proteins; image acquisition on Nikon TE-2000, confocal, deconvolution and high throughput microscopes


Class 4 - Nov. 3, 2011

Lecture

Dr. Bolt - Molecular Cytology: Seeing is Believing (or is it?): Probes, Dyes, Antibodies and Tags

Wet-Lab

Continuation of student projects and training on all scopes; antibodies, epitope tags, XFPs, reporters, experimental design; live and HTM demos; time lapse and photo-bleaching examples


Class 5 - Nov. 10, 2011

Lecture

Dr. Brinkley - Introduction to Electron Optics Electron Microscopy I) and Specimen Preparation (Electron Microscopy II)

Lab

Transmission Electron Microscopy: Training on transmission electron microscope; demonstration of processing and ultra-microtomy (by Brinkley and Townley)

Wet-Lab

Continuation of student projects and training on all scopes


Class 6 - Nov. 17, 2011

Lecture

Dr. Mancini, Dr. Szafran, Dr. Newberg - Automated Microscopy I: Live Cell FRAP and HTM; an overview of recent paradigm shift toward automated microscopy for routine bench science and library screening

Wet-Lab

Continuation of student projects and training on all scopes

More Class Notes


Class 7 - Dec 1, 2011

Lecture

Dr. Mancini, Dr. Szafran, Dr. Newberg - Automated Microscopy II: Image Analyses and Reporting

Wet-Lab

Continuation of student projects and training on all scopes

More Class Notes


Final Exam - Dec. 9, 2011

Dr. Mancini - Final Exam: oral; flexible date dependent on other exams

Oral Exam (~45 min): Discussion with Dr.Mancini and Dr. Brinkley to determine the depth and breadth of "microscopy" acquired during the course

Projects Due: Projects culminate in a PowerPoint presentation of images collected from the labs. Essentially, the PowerPoint show should reflect your accumulated understandings of light and electron microscopy with text and demonstration images from each scope. Example Powerpoint shows from previous classes are available (see below)

Former Student Sample PowerPoints

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