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Genomic and RNA Profiling Core Facility

Houston, Texas

BCM faculty, staff and trainees are the heart of the organization.
Genomic and RNA Profiling Core
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Alternative Amplification Methods for Expression Arrays

The expression array companies do not necessarily endorse the following kits. These possibilities are to be considered if the required starting material specified in the specific array’s protocol cannot be attained.

For Affymetrix 3' GeneChip Arrays:

Various NUGEN kits (including FFPE), see NuGEN Microarray and qPCR Solutions Guide or NuGEN's full website for details.

Ambion MessageAmp:
For Eukaryotic Affymetrix GeneChip Arrays:

MessageAmp II-Biotin-Enhanced Single Round Amplification Kit:
System requires 50ng of intact total RNA (max is 5,000ng, recommended is 1,000ng) in 10uL.

MessageAmpII-Biotin-Enhanced Two Rounds of Amplification Kit: MessageAmpII & MessageAmp II-Biotin-Enhanced Single Round Amplification Kit:
System requires 100pg-50ng intact total RNA


For Affymetrix Whole Transcriptome GeneChip Arrays:

NuGEN's Applause WT-Amp ST:
System requirements vary depending on the kit. See NuGEN Microarray and qPCR Solutions Guide for details.

For more information about NuGEN products, please visit the NuGEN website.


For Agilent Arrays:

NuGEN's Agilent Solution:
System requirements vary depending on the kit. See NuGEN's Agilent Solution Guide for details.


For Bacterial Affymetrix GeneChip Arrays:

MessageAmp-II-Bacteria RNA Amplification Kit:
System requires 100ng-1000ng intact total RNA


For Spotted Arrays (Agilent or NimbleGen):

Amino Allyl MessageAmp II:
System requires 100pg-100ng intact total RNA

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