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Genomic and RNA Profiling Core Facility

Houston, Texas

BCM faculty, staff and trainees are the heart of the organization.
Genomic and RNA Profiling Core
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Facility and Equipment

The Genomic and RNA Profiling Core consists of four sections: the Main Lab (Pre-Amplification room), Post-Amplification Room - Microarray Prep, Post-Amplification Room - Sequencing Prep, and an Intermediate Amplification Room for Mixed Prep. Each room houses the equipment and reagents needed to produce, hybridize, and image arrays, as well as to hybridize and scan microarray and other expression-based assays. We also have separate areas in each of these rooms for Next-Generation Sequencing prep. One area is for the library preparation, cluster generation, and sequencing. We have a separate area that houses the data analysis instrumentation.

Descriptions of the equipment in each room

Equipment used in the Genomic and RNA Profiling Core:

  • Agilent 2100 Bioanalyzer (Lab-On-A-Chip) - RNA Quality Assurance (SAQC)
  • NanoDrop® ND-1000 Spectrophotometer and NanoDrop 2000 (Thermo Scientific)- RNA Quality Assurance (SAQC)
  • Four PTC-100 Programmable Thermal Controllers (MJ Research)
  • PTC-200 DNA Engine and a Dual Head PCT-200 DNA Engine (MJ Research)
  • DNA Engine Tetrad™ Thermal Cycler made by MJ Research
  • Agilent SureCycler 8800
  • Invitrogen Qubit® and a Qubit® 2.0 - RNA Quality Assurance (SAQC)
  • Affymetrix Hybridization Oven 640 - Affymetrix GeneChip® Hybridization (AFFY)
  • GeneChip® Fluidics Station 450 - Affymetrix GeneChip® Washing and Staining (AFFY)
  • GeneChip® Scanner 3000 - Affymetrix GeneChip® Imaging (AFFY)
  • Agilent Microarray Scanner - Imaging for all Agilent arrays as well as other printed slide arrays (cDNA)
  • Ozone Solution Ozone Interceptor which destroys ambient ozone within a benchtop working chamber
  • Aeroqual’s Ozone Monitor NT-70
  • MAUI 4-bay Hybridization Station - hybridization for NimbleGen arrays
  • Bio-Rad Gel Doc-Imaging electrophoretic gels (SAQC)
  • Bio-Rad iCycler iQ Real Time PCR Detection System - Quantitative PCR Detection - For SuperArray processing ONLY!
  • Luminex 200 System
  • Labnet Vortemp 56
  • Perkin Elmer Victor X3 Plate Reader
  • SpectraMax 384 Plus Plate Reader
  • Illumina cBot
  • Illumina HiSeq 2000
  • Illumina MiSeq (coming late October)
  • NanoString - nCounter Prep Station 5s
  • NanoString - nCounter Digital Analyzer 5s
  • Sage Science Pippen Prep
  • Covaris S2
  • Applied Biosystems ViiA7 qPCR (Life Technologies) - for qPCR and Applied Biosystems Microfluidics Array Cards
  • Thermo Scientific Megafuge 40 Centrifuge - Microfluidics Array Cards Rotor only
  • Eppendorf Centrifuge 5810
  • Beckman Coulter Microfuge 22R Centrifuge (for centrifugation at 4oC)
  • Eppendorf Vacufuge and Vacufuge Plus

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