The following is the procedure for In vitro fertilization:
1. Schedule the IVF with the GEM Core.
2. Submit a request to have the mouse (mice) transferred by CCM to R119.
3. Mark clearly on the transfer request that the mice are for IVF and must be transferred the same day as the scheduled request for IVF and are required to be in place by 7 a.m.
4. The core will collect sperm and freeze into nine straws. An IVF will be performed on WT eggs and check for 2-cell embryos as quality control.
Any questions should be directed to Memsmail@bcm.edu.