Genetically Engineered Mouse Core
Transgenic Mouse Core
Main Menu
 
Home

Mission

Services

Protocol

Scheduling

MEMS

Links

Atlas

Personnel

Contacts

 

DNA Microinjection

The GEM Core will microinject traditional transgene DNA constructs or BAC DNA into the one cell mouse embryo. The core injects 150 to 200 embryos per scheduled injection. It provides the investigators with the pregnant recipient moms or the weaned pups depending upon their research needs, transient transgenic or the establishment of transgenic mouse lines, respectively. The GEM core routinely generates transgenic mice in the C57BL/6 and FVB/N background. The Core will microinject DNA into special mouse strains depending on the ability to harvest embryos from those strains. All mice are generated specific pathogen free in B3 housing in the Transgenic Mouse Facility.


Home