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Thank you for your interest in our CCSC Analyzer Training Course. Please email email@example.com and we will e-mail you when space becomes available in our Analyzer Training Course. If you need analysis or sorting done before the next training class, there are technicians here that can assist you in running your samples. Click here for an overview of how to request these services.
This Analyzer Training instructs on the LSRII, LSRFortessa and CantoII. The Analyzer Training Course consists of a lecture and a lab. All students must be available for both the lecture and the lab in the same week. The lecture is offered on a Tuesday from 9 a.m. - 2 p.m. The lab is the following Wednesday from 9 a.m. - 12:30 p.m.. (This training will allow you 24-hour access for Cytometric Analysis of your samples whenever needed. This training is not for unassisted sorting on our FACSAria, FACSAriaII, or assisted analysis.)
The pre-requisite for the Analyzer Training Course is the pre-course required reading "Introduction to Flow Cytometry" workbook. These pre-course materials are in the email that invites you to the course. The questions in the booklet must be answered and turned in at the beginning of the class on Monday.
All course materials and samples are provided (do not bring your own cells). The total cost for Analyzer Training is $225 for internal BCM users.
(If you fail to show up for class, you will be charged a no-show fee. If you are confirmed for a class and need to cancel, you must cancel no later than the Thursday before the start of class to avoid the charge for the course. Also, if you do not complete the pre-training requirements before class, you will be not be able to take the class and you will be charged the full amount.)
- Register in iLab Calendar system.
- Complete the CCSC User Form (PDF)*
- Read "Introduction to Flow Cytometry: A Learning Guide." (PDF)
- Read "CCSC Policies" (PDF)
- If you have not completed the pre-training reading assignment, you will not be able to take the training class and you will still be charged the fee.
- Pre-training and training on the analyzers are required before users can operate these instruments independently.
- Analyzer training classes are scheduled depending on demand. They are typically on Mondays, 9 a.m. - 12 p.m., Tuesdays, 9 a.m. - 1 p.m., and Wednesdays, 9 a.m. - 1:30 p.m.
- Class size is limited to 4-6 students.
- All users must show proficiency on the analyzers by taking the Wednesday portion of the class.
- Those who feel they need a refresher should take the Wednesday class. Please email ccsc to reserve a slot in the class.
Consultation regarding your experimental design, instrument operation, and setup for acquisition or data analysis is available; simply request an appointment by emailing firstname.lastname@example.org.
- Sign up using the iLab calendar.
- If you finish earlier than your end time, log out and notify us in 1 of 3 ways: 1) add a comment to the reservation on the calendar saying to shorten your time or 2) send an e-mail to the staff with the date, original time of the appointment and corrected end time or 3) tell a technician if during CCSC business hours.
- Please cancel ASAP. Users CANNOT CANCEL reserved time on the instrument AFTER the time has started. You will be billed if you do not cancel in advance. If your time has already started, call us at 713-798-3868 to have us end your time that has not passed.
Your First Visit (some helpful suggestions)
- Confirm experimental design (controls etc.) with CCSC staff.
- Sign up for assistance with first run. The analyzer training course comes with a free 60 minutes of assistance after completion - subject to the staff's availability. Please email to schedule.
- Start with no more than ~10 samples in 1.0-2.0 ml volume (0.5-5.0 x 106 cells or more).
- Cells should be in suspension with no obvious clumps. Filtering is a must. You can purchase 35um filter-cap tubes from the CCSC (25 tubes for $25 charged to your cost center).
- Samples should be brought to the core in BD FACS tubes, polystyrene only. The following are item numbers for tubes that are acceptable: (not a comprehensive list)
-352052 (5 mL tubes with no lid)
-352054 (5 mL tubes with lids)
-21008-948 (5 mL filter-cap tubes)
- Samples can also be run from 96 well plates on our high throughput system (HTS). E-mail email@example.com for more information.
Cell populations to be analyzed on any of the instruments cannot contain radionuclides or infectious agents. Specifically, it is not permissible to bring cells into the Cytometry Core for analysis that contain radioactive substances (eg, P-32) or are known to be infected with bacteria, viruses, or fungi. Examples would include cells known to be producing infectious virus such as EBV or HIV. This policy is enforced because flow cytometers generate aerosols, which we cannot contain during analysis or sorting.
- Check out individual analyzer start-up/shutdown procedures at our protocols page.
- Shut down the cytometer and computer if you are the last user of the day.
- Supply reservoir tank should be FULL and waste reservoir tank should be EMPTY for the next user.