Frequently Asked Questions
Frequently used links:
What are your prices for sorting?
We charge $100/hour for an assisted sort. You can find this and other pricing information on our Instrumentation Fee Page.
How do I prepare my sample?
Read the Sorting Guideline (PDF).
How long will my appointment take?
It depends. Things that will affect this would be how many samples there are to analyze, the concentration of the samples, the number of events needing to be collected for each sample, the assay being performed (e.g. cell cycle tends to take a little longer as you need to run the samples in LO), and how many fluorophores are being used. Ask a technician for an estimate.
How many cells will I get back from my cell sorting appointment?
It depends. Things that will affect this would be how many samples there are to sort, the concentration of the samples, the number of cells in the samples, the percentage positive (or the percentage of the gate) that you want to sort, the number of cells you want to collect for each sample, the amount of time of the appointment, and what purity setting you wish the cells to be sorted at. Ask a technician for an estimate.
How do I get training on your equipment?
You can view the information on our Training page.
How do I get trained to sort?
What type of tubes do I use for analysis?
BD FACS tubes, polystyrene only. The following are item numbers for tubes that are acceptable: (not a comprehensive list)
- 352052 (5 mL tubes with no lid)
- 352054 (5 mL tubes with lids)
- 21008-948 (5 mL filter-cap tubes) - 35um mesh filter
What type of tubes do I use for sorting?
For samples, we can use BD Falcon 15 mL conicals, any 5 mL tube, or 1.1 mL tubes.
For collection, you may use 15 mL (any), 5 mL (any), or 1.5 mL (any). You may also use plates and slides of any brand.