About the Core
Continuing the tradition of innovative collaboration with Baylor College of Medicine, the Robert J. Kleberg, Jr. and Helen C. Kleberg Foundation has generously donated $2.26 million to enable the establishment of the Cell-Based Assay Screening Service (C-BASS) core facility, housed in the Verna and Marrs McLean Department of Biochemistry and Molecular Biology.
By providing access to professional expertise and a variety of cutting-edge technologies and robotics platforms, the C-BASS core facility seeks to facilitate the advancement of our understanding of complex biological questions using well-established model systems, and to foster collaborations amongst BCM researchers.
Our links for in-house database search are not available right now. Please follow the directions below for ordering through the iLab Portal.
We are transitioning to the iLab ordering system and fixing the database search issues. We apologize for all the inconveniences and hope to resolve the problems as quickly as possible. In the meantime, please follow the following steps for all your orders.
(1) For GIPZ shRNA clone requests: Go to http://dharmacon.gelifesciences.com/openbiosystems/, and search for your gene of interest (for mouse or human). Select the individual clones that you want (based on hairpin sequence information etc.), and copy down the clone ID#. Then go to the iLab portal-- Use your BCM credentials to log into iLab. Enter the gene name and clone ID# either DIRECTLY INTO THE TABLE or DOWNLOAD THE FORM from iLab and then enter the information in the form before uploading it.
(2) For cDNA clone requests: Go to the iLab portal, and enter the gene information, either DIRECTLY INTO THE TABLE or DOWNLOAD THE FORM from iLab and then enter the information in the form before upload it through the iLab portal.
(3) For equipment reservation: Go to the iLab portal.
For pricing information on all services other than CRISPR, see C-BASS Price Schedule - 2015. For equipment use, please go to the iLab portal, to access automated microscopes, robotic workstations, and LSRII with HTS. Register first if new user.
CRISPR/Cas9 Genome-Editing Services
For generating knockout (KO) or knock-in (KI) cell lines, investigators can choose services that suit their needs.
We offer full CRISPR vector construction services (3-4 gRNA constructs), and will verify cutting efficiencies of the constructs.
C-BASS is also equipped to work with investigators on further isolation, verification, and analysis of CRISPR KO/KI clones.
See the vector list. See the Price List for Custom CRISPR KO/KI cell line services.
If you would like to generate KO/KI cells on your own, here is a detailed protocol from Dr. Feng Zhang's group.
Platforms for Cell-Based Assays
We aim to facilitate the use of various genomic reagents in screens, in studies of specific pathways or individual genes, and we provide in-house, high-throughput image analysis and flow cytometry.
Functional Genomics using shRNA and cDNA libraries
Bi-Molecular Fluorescence Complementation Assay
Services for studying protein-protein interactions in live cells using the Bi-Molecular Fluorescence Complementation (BiFC) Assay. In the BiFC assay, proteins X and Y are respectively tagged with split GFP fragments (GFPn and GFPc). X-Y interaction enables co-folding of GFPn and GFPc and fluorescence complementation, which can be detected by FACS. BIFC is particularly suitable for detecting transient, low affinity, or low abundance interactions.
We will assist investigators in utilizing the split GFP technology to examine protein-protein interactions in live cells.
C-BASS houses Automated Workstations that can facilitate potential drug screens and limiting dilution assays. View information on our workstations.