About the Core
Continuing the tradition of innovative collaboration with Baylor College of Medicine, the Robert J. Kleberg, Jr. and Helen C. Kleberg Foundation has generously donated $2.26 million to enable the establishment of the Cell-Based Assay Screening Service (C-BASS) core facility, housed in the Verna and Marrs McLean Department of Biochemistry and Molecular Biology.
By providing access to professional expertise and a variety of cutting-edge technologies and robotics platforms, the C-BASS core facility seeks to facilitate the advancement of our understanding of complex biological questions using well-established model systems, and to foster collaborations amongst BCM researchers.
CRISPR/Cas9 Genome-Editing Services
For generating knockout (KO) or knock-in (KI) cell lines, investigators can choose services that suit their needs.
We offer CUSTOM CRISPR vector construction services ($200/construct). We can also verify cutting efficiencies for a small fee.
C-BASS is also equipped to work with investigators on further isolation, verification, and analysis of CRISPR KO/KI clones.
If you would like to generate KO/KI cells on your own, here is a detailed protocol from Dr. Feng Zhang's group.
Functional Genomics using shRNA and cDNA libraries
(1) For GIPZ shRNA clone requests: Go to http://dharmacon.gelifesciences.com/openbiosystems/, and search for your gene of interest (for mouse or human). Select the individual clones that you want (based on hairpin sequence information etc.), and copy down the clone ID#. Then go to the iLab portal-- Use your BCM credentials to log into iLab. Enter the gene name and clone ID# either DIRECTLY INTO THE TABLE or DOWNLOAD THE FORM from iLab and then enter the information in the form before uploading it.
If you are unclear about how to select shRNA clones, please email us at firstname.lastname@example.org with the name of your gene and we will help you with the process.
(2) For cDNA clone requests: Go to the iLab portal, and enter the gene information, either DIRECTLY INTO THE TABLE or DOWNLOAD THE FORM from iLab and then enter the information in the form before upload it through the iLab portal.
All shRNA/cDNA clones are $45 each and distributed as bacterial glycerol stocks in individual tubes. For orders of multiple clones (e.g., >30), clones may be arrayed in multi-well plates for distribution. We do offer discounts for orders of >50 clones or more.
Bi-Molecular Fluorescence Complementation Assay
We also offer support services for studying protein-protein interactions in live cells using the Bi-Molecular Fluorescence Complementation (BiFC) Assay. In the BiFC assay, proteins X and Y are respectively tagged with split GFP fragments (GFPn and GFPc). X-Y interaction enables co-folding of GFPn and GFPc and fluorescence complementation, which can be detected by FACS. BIFC is particularly suitable for detecting transient, low affinity, or low abundance interactions.
We will assist investigators in utilizing the split GFP technology to examine protein-protein interactions in live cells.