- Department of Molecular and Cellular Biology Baylor College of Medicine One Baylor Plaza M725A Houston, Texas 77030 United States
- Baylor College of Medicine
- BS from Cornell University
- MS from Michigan State University
- PhD from Michigan State University
- Post-Doctoral Fellowship at Baylor College of Medicine
- Molecular regulation of cellular differentiation and physiology
The goal of my laboratory is to investigate the molecular regulation of cellular differentiation and physiology. This research is conducted on two model organ systems, the lung and uterus. Although these two tissues are significantly divergent in their biological functions, many of the molecular mechanisms regulating the cellular differentiation and physiology are conserved. In order to investigate the biology of these tissues, my laboratory has manipulated the mouse genome to generate novel animal models to identify molecular mechanisms regulating the cell biology of these organs.
The lung is composed of 40 different cell types. This makes the lung an interesting organ to investigate the developmental control of cellular differentiation. The pulmonary cell types my laboratory is interested in investigating are the Clara cells, the neuroendocrine cells and the alveolar type II cells. Clara cells are the non-ciliated secretory cells of the pulmonary epithelium. My laboratory has used transgenic technology to execute in vivo promoter analysis to investigate the molecular regulation of Clara cell gene expression. The information gained from these studies has allowed us to generate an animal model for lung cancer, to generate cell lines to further investigate the elements regulating Clara cell differentiation and finally to determine how elements involved in lung development play a role in the regulation of the response of the Clara cell to environmental challenges. In the investigation of the factors that control neuroendocrine cell differentiation, my laboratory is interested in identifying what factors regulate this process as well as determining the role of these cells in damage and repair of the pulmonary epithelium. We have shown that the transcription factor, achaete scute, can cause a transformed Clara cell to express markers of neuroendocrine differentiation in vivo. Finally, in the investigation of the biology of the alveolar type II cell my laboratory has developed an transgenic “Gene Switch” system to investigate how growth factors which are involved in regulating lung development can function to regulate the biology of the alveolar type II cell in the adult.
The uterus functions to support the development of the fetus. The ability of the embryo to attach and thrive in the uterus is under tight hormonal control. Ablation of the receptor for the steroid hormone progesterone has demonstrated that this hormone is critical for the uterus to initiate and support the implanting embryo. My laboratory is interested in understanding the cascade of events regulated by progesterone. This is being accomplished by using current techniques in gene expression analysis to determine which genes are regulated by progesterone. Finally my laboratory is generating novel approaches to investigate the role of specific genes in uterine biology in vivo.
The overall goal of the above investigations in the understanding of the molecular regulation of cellular differentiation and physiology is to shed light on pathways to aid in the diagnosis and treatment of human disease. Understanding the molecular regulation of pulmonary cell differentiation will help design treatments for pulmonary diseases such as lung cancer, and asthma. The investigation of uterine biology will aid in the treatment of infertility.
- Rubel CA, Lanz RB, Kommagani R, Franco HL, Lydon JP, Demayo FJ. "Research resource: genome-wide profiling of progesterone receptor binding in the mouse uterus.." Mol. Endocrinol.. 2012 August;26(8):1428-42. Pubmed PMID: 22638070
- Franco HL, Rubel CA, Large MJ, Wetendorf M, Fernandez-Valdivia R, Jeong JW, Spencer TE, Behringer RR, Lydon JP, Demayo FJ. "Epithelial progesterone receptor exhibits pleiotropic roles in uterine development and function.." FASEB J.. 2012 March;26(3):1218-27. Pubmed PMID: 22155565
- Franco HL, Dai D, Lee KY, Rubel CA, Roop D, Boerboom D, Jeong JW, Lydon JP, Bagchi IC, Bagchi MK, Demayo FJ. "WNT4 is a key regulator of normal postnatal uterine development and progesterone signaling during embryo implantation and decidualization in the mouse.." FASEB J.. 2011 April;25(4):1176-87. Pubmed PMID: 21163860
- Jeong JW, Kwak I, Lee KY, Kim TH, Large MJ, Stewart CL, Kaestner KH, Lydon JP, DeMayo FJ. "Foxa2 is essential for mouse endometrial gland development and fertility.." Biol. Reprod.. 2010 September;83(3):396-403. Pubmed PMID: 20484741
- Franco HL, Lee KY, Rubel CA, Creighton CJ, White LD, Broaddus RR, Lewis MT, Lydon JP, Jeong JW, DeMayo FJ. "Constitutive activation of smoothened leads to female infertility and altered uterine differentiation in the mouse.." Biol. Reprod.. 2010 May;82(5):991-9. Pubmed PMID: 20130264
- Franco HL, Lee KY, Broaddus RR, White LD, Lanske B, Lydon JP, Jeong JW, DeMayo FJ. "Ablation of Indian hedgehog in the murine uterus results in decreased cell cycle progression, aberrant epidermal growth factor signaling, and increased estrogen signaling.." Biol. Reprod.. 2010 April;82(4):783-90. Pubmed PMID: 20056671
- Jin N, Cho SN, Raso MG, Wistuba I, Smith Y, Yang Y, Kurie JM, Yen R, Evans CM, Ludwig T, Jeong JW, DeMayo FJ. "Mig-6 is required for appropriate lung development and to ensure normal adult lung homeostasis.." Development. 2009 October;136(19):3347-56. Pubmed PMID: 19710174
- Jeong JW, Lee HS, Lee KY, White LD, Broaddus RR, Zhang YW, Vande Woude GF, Giudice LC, Young SL, Lessey BA, Tsai SY, Lydon JP, DeMayo FJ. "Mig-6 modulates uterine steroid hormone responsiveness and exhibits altered expression in endometrial disease.." Proc. Natl. Acad. Sci. U.S.A.. 2009 May 26;106(21):8677-82. Pubmed PMID: 19439667
- Jeong JW, Lee HS, Franco HL, Broaddus RR, Taketo MM, Tsai SY, Lydon JP, DeMayo FJ. "beta-catenin mediates glandular formation and dysregulation of beta-catenin induces hyperplasia formation in the murine uterus.." Oncogene. 2009 January 8;28(1):31-40. Pubmed PMID: 18806829
- Li H, Cho SN, Evans CM, Dickey BF, Jeong JW, DeMayo FJ. "Cre-mediated recombination in mouse Clara cells.." Genesis. 2008 June;46(6):300-7. Pubmed PMID: 18543320