Michael Robichaux, Ph.D.
Michael Robichaux, Ph.D.
Postdoctoral Fellow - Mentor: Ted Wensel, Ph.D.
- Postdoctoral Fellow - Mentor: Ted Wensel, Ph.D.
Baylor College of Medicine
Houston, Texas United States
- Baylor College of Medicine (Lab)
BCM-Alkek Graduate School
Houston, TX 77030
- BS from Nicholls State University
- 05/2008 - Thibodaux, Louisiana United States
- Biology (Pre-Medicine)
- PhD from University of Texas Southwestern Medical Center
- 05/2013 - Dallas, Texas United States
- Post-Doctoral Fellowship at Baylor College of Medicine
- Houston, Texas United States
- Biochemistry and Molecular Biology
- Cell Biology and Cell Fate Determination
- Cryo-Electron Microscopy and Tomography
- Membrane Trafficking and Cytoskeleton
- Protein Structure-Function and Protein Folding
- Signal Transduction
- Structural Biology and Macromolecular Assemblies
- Mouse and Rat
- Xenopus and Zebrafish
Professional StatementLOCALIZATION OF THE BARDET-BIEDL SYNDROME PROTEIN COMPLEX IN ROD PHOTORECEPTOR CILIA
Michael Robichaux, Theodore G. Wensel
Bardet-Biedl syndrome (BBS) is a pleiotropic ciliopathy characterized by the dysfunction of primary cilia and retinal degeneration of the photoreceptor cells of the retina, which, among other disease outcomes, leads to vision loss and blindness. 8 essential BBS ciliary proteins form the macromolecular BBSome protein complex, which functions as a membrane coat in vitro and is hypothesized to regulate of key trafficking events within the primary cilia of most cell types. Beyond preliminary findings, however, little is known of the macromolecular structure of the BBSome or its precise function in primary cilia, including the rod outer segment (ROS) sensory cilium of the retina. One key limitation to our understanding of the retinal BBSome is the lack of sublocalization data within the small area of the ROS cilia in photoreceptor cells. Thus, I have begun a rigorous sublocalization analysis of the BBSome complex in ROS sensory cilia using high-powered STORM super resolution imaging, which enables the localization of the retinal BBSome proteins to a resolution of approximately 20-30 nm. At such a highresolution, I have been able to confirm the localization of BBSome proteins near the basal body complex and surrounding the restrictive connecting cilium portion of the ROS cilium. However, to improve this STORM super resolution analysis and complete my analysis of the retinal BBSome, I have developed a method for creating homemade STORM antibodies that are specially conjugated to multiple STORM-specific photoswitching fluorophores. I have demonstrated that this novel immunostaining reagent that I specifically designed for STORM imaging will yield more accurate STORM reconstructions than those generated using commercial reagents. Together, these early and proposed sublocalization findings will greatly inform future functional studies of the BBSome and contribute to our current understanding of Bardet-Biedl syndrome and other crippling retinal ciliopathies.
- Robichaux MA1, Chenaux G, Ho HY, Soskis MJ, Dravis C, Kwan KY, Šestan N, Greenberg ME, Henkemeyer M, Cowan CW "EphB receptor forward signaling regulates area-specific reciprocal thalamic and cortical axon pathfinding." Proceedings of the National Academy of Scienc. 2014 February 11; 111 (6): 2188-93. Pubmed PMID: 24453220
- Srivastava, N.*, Robichaux, M.A.*, Chenaux, G., Henkemeyer, M., and Cowan, C.W "EphB2 receptor forward signaling controls cortical growth cone collapse via Nck and Pak." Mol Cell Neurosci. 2013 January ; 52 : 106-16. Pubmed PMID: 23147113
- Soskis, M.J., Ho, H.Y., Bloodgood, B.L., Robichaux, M.A., Malik, A.N., Ataman, B., Rubin, A.A., Zieg, J., Zhang, C., Shokat, K.M., Sharma, N., Cowan, C.W., Greenberg, M.E "A chemical genetic approach reveals distinct EphB signaling mechanisms during brain development." Nat Neurosci. 2012 December ; 15 (12): 1645-54. Pubmed PMID: 23143520
- Robichaux, M. A., and Cowan, C. W "Signaling Mechanisms of Axon Guidance and Early Synaptogenesis. In Current Topics in Behavioral Neurosciences." Current Topics in Behavioral Neurosciences: T. 2014 16 : 19-48. Pubmed PMID: 24318963
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