Next-Generation Sequencing Services
Due to the increased demand for rapid DNA sequencing, the facility now provides massively parallel “next generation” sequencing on the Illumina HiSeq 2000, the Illumina MiSeq and Illumina HiSeq 2500. Our core lab has established a workflow involving: project consultation, sample quality check, sample preparation and data generation for each sequencing project. Illumina’s sequencing platform provides high-quality data in the following applications: gene expression and alternative splicing (mRNA-Seq), protein-nucleic acid association profiling and epigenetics (ChIP-Seq), sequencing targeted genomic regions, small RNA discovery (small RNA-Seq) and de novo sequencing.
The GARP offers investigators access to an array of emerging technologies while assisting in experimental design and data analysis.
GARP offers Next Generation Sequencing Services on the Illumina MiSeq, HiSeq 2000 and HiSeq 2500 Systems. The services include:
The above links will take you to the area of the Illumina website that discusses each application.
*This service provided by the Genomic and RNA Profiling Core Facility, not in conjunction with Illumina.
Illumina's MiSeq and HiSeq systems' powerful combination of read-length and paired-end flexibility enables the broadest range of genomic sequencing applications. Illumina's robust sequencing chemistry supports a wide range of read lengths, allowing researchers to tailor each run to meet their needs with shorter turnover times.
|MiSeq Benchtop Sequencer||HiSeq 2500 Sequencer||HiSeq 2500/2000 Sequencer|
|Mode||-||Rapid Run||High Output|
|Output (Max.)||15 Gb||
90 Gb - Single FC
180 Gb - Dual FC
300 Gb - Single FC
600 Gb - Dual FC
|Run Time||4 - 65 hrs||7 - 40 hrs||2 - 11 days|
|Single End Reads (Max.)||25 Million||
300 Million - Single FC
600 Million - Dual FC
1.5 Billion - Single FC
3 Billion - Dual FC
|Paired End Reads (Max.)||50 Million||
600 Million - Single FC
1.2 Billion - Dual FC
3 Billion - Single FC
6 Billion - Dual FC
|Read Length (Max.)||2 x 300 bp||2 x 150 bp||2 x 100 bp|
|Bases above Q30||>85% (2 x 75 bp)||>80% (2 x 100 bp)||>80% (2 x 100 bp)|
Gb = gigabases, bp = base pair
1 Flow Cell (FC) = 8 Lanes
Read more about GARP's sequencing platforms: