Alternative Amplification Methods for Expression Arrays
The expression array companies do not necessarily endorse the following kits. These possibilities are to be considered if the required starting material specified in the specific array’s protocol cannot be attained.
For Affymetrix 3' GeneChip Arrays:
For Eukaryotic Affymetrix GeneChip Arrays:
MessageAmp II-Biotin-Enhanced Single Round Amplification Kit:
System requires 50ng of intact total RNA (max is 5,000ng, recommended is 1,000ng) in 10uL.
MessageAmpII-Biotin-Enhanced Two Rounds of Amplification Kit: MessageAmpII & MessageAmp II-Biotin-Enhanced Single Round Amplification Kit:
System requires 100pg-50ng intact total RNA
For Affymetrix Whole Transcriptome GeneChip Arrays:
For more information about NuGEN products, please visit the NuGEN website.
For Agilent Arrays:
For Bacterial Affymetrix GeneChip Arrays:
MessageAmp-II-Bacteria RNA Amplification Kit:
System requires 100ng-1000ng intact total RNA
For Spotted Arrays (Agilent or NimbleGen):
Amino Allyl MessageAmp II:
System requires 100pg-100ng intact total RNA