Next-Generation Sequencing Data Services
The GARP Core offers some bioinformatics support for data analysis. Our basic data services currently include:
- The Illumina MiSeq, HiSeq 2000 and HiSeq 2500 pipeline software for the customer’s specific experiment, including image analysis, base calling and sequence alignment steps.
- A detailed report including sequence reads, exon location, exon-intron junction region, gene location and SNP calls.
- Experiment quality parameters such as base quality scores, quality control metrics, and sequencing run statistics.
DNA-Protein Interaction Analysis (ChIP-seq)The MiSeq, HiSeq 2000 and HiSeq 2500 also offer powerful DNA-Protein interaction analysis. By combining chromatin immunoprecipitation with Illumina’s sequencing technology (CHIP-seq) on a genome-wide scale, biologists can accurately identify a broad range of interactions, accurately survey interactions between protein, DNA, and RNA to interpret regulation events central to many biological processes and disease states.
Gene Regulation Analysis Whether you are interested in CpG methylation, histone modifications, chromatin structure, or DNA-protein interactions, Illumina's unique sequencing chemistry gives you unbiased coverage of every base in the genome. Use Illumina's ChIP-seq protocol to generate accurate and precise DNA-protein interaction maps from a single chromatin immunoprecipitated sample. Map genome-wide methylation patterns by sequencing bisulfite-converted DNA, methylation-sensitive restriction digest-enriched fragments, anti-methyl-C precipitated fragments, or chromatin immunoprecipitates of methyltransferases trapped to aza-labeled DNA.
Transcriptome Analysis The MiSeq, HiSeq 2000 and HiSeq 2500 make it possible for biologists to characterize all transcriptional activity, coding and non-coding, in any organism without a priori assumptions and achieve an unprecedented view of the transcriptome.By combining long and short reads, strand specificity, and millions of counts, Illumina allows you to annotate coding SNPs, discover transcript isoforms, identify regulatory RNAs, characterize splice junctions, and determine the relative abundance of transcripts.
SNP Discovery and Structural Variation AnalysisIllumina's MiSeq, HiSeq 2000 and HiSeq 2500 generate the highest quality data available to analyze any genome for any species, and every genetic variant. A flexible, convenient workflow allows you to combine both short-insert paired-end and long-insert mate pair libraries to create an optimal level of detail and accuracy for any study. Go rapidly from DNA to data with minimal hands-on effort to produce meaningful results, reliably and efficiently.
Gene Regulation and Epigenetic Analysis/Sequence-Based Methylation AnalysisIdentify and track methylation patterns by directly sequencing bisulfite-converted DNA, methylation-sensitive restriction digest-enriched fragments, anti-methyl C-precipitated fragments, or chromatin immunoprecipitates of methyltransferases trapped to aza-labeled DNA on the MiSeq, HiSeq 2000 and HiSeq 2500. Correctly map more reads to the methylated genome using accurate 100+ base paired-end reads. Easily prepare highly diverse libraries needed for comprehensive characterization of methylation status. Using Illumina sequencing technology, you can evenly sequence a repetitive bisulfite-converted genome and detect variations in methylation signatures at single-base resolution to pinpoint rare binding events to within 50 bases of the actual binding site.