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Genomic and RNA Profiling Core Facility

Houston, Texas

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Genomic and RNA Profiling Core
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Next-Generation Sequencing Services

Due to the increased demand for rapid DNA sequencing, the facility now provides massively parallel “next generation” sequencing on the Illumina HiSeq 2000, the Illumina MiSeq and Illumina HiSeq 2500. Our core lab has established a workflow involving: project consultation, sample quality check, sample preparation and data generation for each sequencing project. Illumina’s sequencing platform provides high-quality data in the following applications: gene expression and alternative splicing (mRNA-Seq), protein-nucleic acid association profiling and epigenetics (ChIP-Seq), sequencing targeted genomic regions, small RNA discovery (small RNA-Seq) and de novo sequencing.

The GARP offers investigators access to an array of emerging technologies while assisting in experimental design and data analysis.

GARP offers Next Generation Sequencing Services on the Illumina MiSeq, HiSeq 2000 and HiSeq 2500 Systems. The services include:

Next-Generation Sequencing Services
DNA Sequencing DNA-Protein Interaction Analysis (ChIP-Seq)
Gene Expression Analysis Sequencing-Based Methylation Analysis
Sequencing-Based Transcriptome Analysis Small RNA Discovery
Targeted (Amplicon) Resequencing Analysis Microbial Sequencing Analysis
Targeted RNA Expression Analysis FFPE Transcriptome Analysis and Low-Quality RNA-Seq Analysis

The above links will take you to the area of the Illumina website that discusses each application.

*This service provided by the Genomic and RNA Profiling Core Facility, not in conjunction with Illumina.

Read Lengths

Illumina's MiSeq and HiSeq systems' powerful combination of read-length and paired-end flexibility enables the broadest range of genomic sequencing applications. Illumina's robust sequencing chemistry supports a wide range of read lengths, allowing researchers to tailor each run to meet their needs with shorter turnover times.

Illumina Sequencing Instrument Specifications

MiSeq Benchtop Sequencer HiSeq 2500 Sequencer HiSeq 2500/2000 Sequencer
Mode - Rapid Run High Output
Output (Max.) 15 Gb

90 Gb - Single FC

180 Gb - Dual FC

300 Gb - Single FC

600 Gb - Dual FC

Run Time 4 - 65 hrs 7 - 40 hrs 2 - 11 days
Single End Reads (Max.) 25 Million

300 Million - Single FC

600 Million - Dual FC

1.5 Billion - Single FC

3 Billion - Dual FC

Paired End Reads (Max.) 50 Million

600 Million - Single FC

1.2 Billion - Dual FC

3 Billion - Single FC

6 Billion - Dual FC

Read Length (Max.) 2 x 300 bp 2 x 150 bp 2 x 100 bp
Bases above Q30 >85% (2 x 75 bp) >80% (2 x 100 bp) >80% (2 x 100 bp)

Gb = gigabases, bp = base pair

1 Flow Cell (FC) = 8 Lanes

Click the following links to learn more about these instruments: MiSeq Benchtop Sequencer, HiSeq 2000 Sequencer, HiSeq 2500 Sequencer.

Read more about GARP's sequencing platforms:

mRNA and Total RNA Sequencing

ChIP Sequencing

DNA Sequencing

miRNA or miRNA-Small RNA Sequencing

Covaris Shearing Services

Sequencing Data Services

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