John L. Spudich, Ph.D.
Professor and Robert A. Welch Distinguished Chair in Chemistry
Professor, Biochemistry and Molecular Biology
Director, Center for Membrane Biology
University of Texas-Houston Medical School
B.S. Mathematics, Univ Illinois, Urbana (1967)
Ph.D. Biophysics, Univ California, Berkeley (1976)
Harvard Medical School, Boston (1976-1978) University of California, San Francisco (1978-1980)
The primary interest in Dr. Spudich's laboratory is the mechanism by which photosensory receptors sense and transmit information concerning the color, intensity, and pattern of light in the environment. They study a widespread class of photoactive receptor proteins (rhodopsins) that consist of seven transmembrane helices connected by interhelical loops. The helices form a pocket for the photosensitive molecule vitamin-A aldehyde (retinal), and the receptor proteins physically couple to protein transducers that relay signals to sensory pathways in the cytoplasm. These photosensitive seven helix proteins are used for visual processes of various degrees of sophistication, ranging from detection of light-dark boundaries,light gradients, and light direction by single-cell microorganisms to high-resolution color image detection by animal eyes.
Sineshchekov OA, Govorunova EG, Wang J, Li H and Spudich JL. Intramolecular proton transfer in channelrhodopsins. Biophys J, 104(4):807-17 (2013). PubMed
Sineshchekov OA, Govorunova EG, Wang J and Spudich JL. Enhancement of the Long-Wavelength Sensitivity of Optogenetic Microbial Rhodopsins by 3, 4-Dehydroretinal. Biochemistry, 51(22):4499-506 (2012). PubMed
Spudich EN, Ozorowski G, Schow EV, Tobias DJ, Spudich JL and Luecke H. A transporter converted into a sensor, a phototaxis signaling mutant of bacteriorhodopsin at 3.0 A. J Mol Biol, 415(3):455-63 (2012). PubMed
Hou SY, Govorunova EG, Ntefidou M, Lane CE, Spudich EN, Sineshchekov OA and Spudich JL. Diversity of Chlamydomonas channelrhodopsins, Photochem Photobiol, 88(1):119-28 (2011). PubMed
Wu M, Tong S, Gonzalez J, Jayarama V, Spudich JL and Zheng L. Structural basis of the Ca2+ inhibitory mechanism of Drosophilia Na+/Ca2+ exchanger CALX and its modification by alternative splicing. Structure, 19(10):1509-17 (2011). PubMed
Govorunova EG, Spudich EN, Lane CE, Sineshchekov OA and Spudich JL. New channelrhodopsin with a red-shifted spectrum and rapid kinetics from Mesostigma viride. MBio, 2(3):e00115-11 (2011). PubMed
Sasaki J, Takahashi H, Furutani Y, Kandori H and Spudich JL. Sensory rhodopsin-I as a bidirectional switch: opposite conformational changes from the same photoisomerization. Biophys J., 100(9):2178-83 (2011). PubMed
For more publications, see listing on PubMed.
Department: Biochemistry & Molecular Biology
Address: 6431 Fannin St., JFB 1.708
UT Houston Medical School
University of Texas Health Science Center
Houston TX 77030
Additional Links: University of Texas-Houston Medical School, Center for Membrane Biology