!

COVID-19 Response 

Access our COVID-19 Response homepage, with more information and resources during the COVID-19 pandemic, including what to do if you’re experiencing symptoms.

Weather Update

Baylor College of Medicine will be open Wednesday and Baylor Medicine will be seeing patients. Please prioritize your safety and take appropriate safety precautions if driving in any high water. View message. 

Department of Pediatrics

Antigen Discovery

Master
Content

The Antigen Discovery and Molecular Biology Unit expresses and purifies recombinant protein antigens for vaccines against neglected tropical diseases and other emerging infectious diseases. The unit focuses on identification and cloning of the molecules secreted by parasites that play important roles in the establishment of parasitism or survival of the parasites in hosts and evaluating their potential as vaccine candidates.

The unit also develops and optimizes the expression platform for each vaccine candidate protein, mainly using yeast, E. coli, or mammalian cell expression systems; makes research seed stocks based on small scale expression yield and solubility; and expresses and purifies recombinant proteins at the small scale for research purposes. The seed stocks are then transferred to the Process Development Unit for large scale production of vaccine antigens. In addition, the Antigen Discovery unit also maintains the hookworm life cycle in hamsters for pre-clinical vaccine trials and drug screening purposes.

Heading

Laboratory Facilities

Content

Dr. Zhan’s laboratory has extensive capability of molecular cloning and recombinant protein expression using Pichia pastoris, Saccharomyces cerevisiae, Escherichia coli, Baculovirus and mammalian cell culture expression systems, with fermentation capabilities from the 1-10L scale. The facility is capable of molecular cloning, developing and optimizing protein expression and purification processes and immunological assay.

Procedures may include, but are not limited to, PCR amplification, Western blot, ion exchange chromatography (anion or cation), hydrophobic interaction chromatography, hydroxyapatite, reverse phase and size exclusion, IMAC and affinity chromatography.

Heading

Unit Director

Content

Bin Zhan, M.D., M.S.

Heading

Research Associate

Content

Junfei Wei, M.D., Ph.D.

Media Component
Media
Heading

Roundworm Antigen Discovery

Content

Roundworms infect an estimated 800 million people around the world.