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Path-Immuno - James Versalovic Lab

Houston, Texas

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Path-Immuno - James Versalovic Lab
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Peer-Reviewed Publications

Probiotics and the Human Microbiome

Preidis GA, Versalovic J. Targeting the human microbiome with antibiotics, probiotics, and prebiotics: Gastroenterology enters the metagenomics era. Gastroenterol 2009;136(6):2015-2031.

Britton RA, Versalovic J. Probiotics and gastrointestinal infections. Interdisciplinary Perspectives on Infectious Diseases. In press.

Saulnier D.M.A, Spinler J.K., Gibson G.R. and Versalovic J. (2009). Mechanisms of Probiosis and Prebiosis: Considerations for Enhanced Functional Foods. Current Opinion in Biotechnology. 20: 1-7.

Hsieh MH, Versalovic J. (2008) The human microbiome and probiotics: implications for pediatrics. Curr Probl Pediatr Adolesc Health Care: 38:309-27. [NIHMS79400]

Becnel Boyd L, Maynard MJ, Morgan-Linnell SK, Horton LB, Banks LB, Sucgang R, Hamill RJ, Rojo Jimenez J, Versalovic J, Steffen D, Zechiedrich L. (2008) Relationships among ciprofloxacin, gatifloxacin, levofloxacin, and norfloxacin MICs in fluoroquinolone-resistant Escherichia coli clinical isolates. Antmicrob Agents Chemother [Epub ahead of print].

McGuire AL, Colgrove J, Whitney SN, Diaz CM, Bustillos D, Versalovic J. (2008) Ethical, legal, and social considerations in conducting the Human Microbiome Project. Genome Res [Epub ahead of print].

Versalovic J, Iyer C, Lin YP, Huang Y, Dobrogosz W. (2008) Commensal-derived probiotics as anti-inflammatory agents. Microb Ecol Health Dis: 20:86-93.

Lin YP, Thibodeaux CH, Peña JA, Ferry GD, Versalovic J. (2008) Probiotic Lactobacillus reuteri suppress proinflammatory cytokines via c-Jun. Inflamm Bowel Dis: 14:1068-83.

Iyer C, Kosters A, Sethi G, Kunnamakara A, Aggarwal BB, Versalovic J. (2008) Probiotic Lactobacillus reuteri promotes TNF-induced apoptosis in human myeloid leukemia-derived cells by modulation of NF-κB and MAPK signaling. Cell Microbiol: 10:1442-52.

Spinler JK, Taweechotipatr M, Rognerud CL, Ou CN, Tumwasorn, Versalovic J. (2008) Human-derived probiotic Lactobacillus reuteri demonstrate antimicrobial activities targeting diverse enteric bacterial pathogens. Anaerobe: 14:166-171. [NIHMS57384]

Whitehead K, Versalovic J, Roos S, Britton RA. (2008) Genomic and genetic characterization of the bile stress response of probiotic Lactobacillus reuteri ATCC 55730. Applied Environ Microbiol: 74:1812-9. [PMC2268311]

Wall T, Bath K, Britton RA, Jonsson H, Versalovic J, Roos S. (2007) The early response to acid shock in Lactobacillus reuteri involves the ClpL chaperone and a putative cell wall-altering esterase. Appl Environ Microbiol: 73:3924-35. [PMC1932720]

Versalovic J, Relman D. (2006). How bacterial communities expand functional repertoires.PLOS Biology, 4: 2193-2195.

Lin YP, Versalovic J. (2006). Immunoregulation by probiotics. AgroFood Industry Hi-Tech, 17:70-3.

Guarner F, Bourdet-Sicard R, Brandtzaeg P, Gill HS, McGuirk P, van Eden W, Versalovic J, Weinstock JV, Rook GAW. (2006) The hygiene hypothesis re-visited.
Nat Clin Pract Gastroenterol & Hepatol, 3:275-284.

Peña JA, Rogers Ab, Ge Z, Ng V, Li SY, Fox JG, and Versalovic J. (2005) Probiotic Lactobacillus diminishes Helicobacter hepaticus-induced inflammatory bowel disease in IL-10-deficient mice. Infect Immun, 73:912-920.

Peña JA, Li SY, Wilson PH, Thibodeau S, Szary AJ, and Versalovic J. (2004). Genotypic and phenotypic studies of murine intestinal lactobacilli: species differences in mice with and without colitis. Appl and Environ Microb, 70:558-568.

Peña JA and Versalovic J . (2003). Lactobacillus rhamnosus GG decreases TNF- a production in lipopolysaccharide-activated murine macrophages by a contact-independent mechanism. Cellular Microbiology. 5:277-285.

Molecular Microbiology

Versalovic J. Arrays and medical microbiology: e pluribus unum. Archives of Pathology & Laboratory Medicine. Accepted for publication.

Boyanton BL, Luna RA, Fasciano LR, Menne KG, Versalovic J. (2008) DNA pyrosequencing-based identification of pathogenic Candida species by using the internal transcribed spacer 2 region. Arch Pathol Lab Med: 132:667-674.

Zhang WW, Versalovic J. (2007) Expanding the diagnostic capabilities of molecular microbiology by genomic methods. J Mol Diagn 9:572-3.

Luna RA, Fasciano LR, Jones SC, Boyanton BL, Ton TT, Versalovic J. (2007) DNA pyrosequencing-based bacterial pathogen identification in a pediatric hospital setting. J Clin Microbiol: 45:2985-92. [PMC2045277]

Gulley ML, Braziel RM, Halling KC, Hsi ED, Kant JA, Nikiforova MN, Nowak JA, Ogino S, Oliveira A, Polesky HF, Silverman L, Tubbs RR, Van Deerlin VM, Vance GH, Versalovic J. (2007) The College of American Pathologists Molecular Pathology Resource Committee: Clinical laboratory reports in molecular pathology. Arch Pathol Lab Med: 131:852-63.

Yee EL, Palacio H, Atmar RL, Umair S, Kilborn C, Faul M, Gavagan TE, Feigin R, Versalovic J, Neill FH, Panlilio AL, Miller M, Spahr J, Glass RI. (2007) Widespread outbreak of Norovirus gastroenteritis among evacuees of Hurricane Katrina residing in a large “mega-shelter” in Houston, Texas: lessons learned for prevention. Clin Infect Dis: 44(8): 1032-1039.

Hulten KG, Kaplan SL, Gonzalez BE, Hammerman W, Lamberth L, Versalovic J, and Mason EO. (2006). Three year surveillance of community-onset healthcare-associated Staphylococcus aureus infections in children. Pedi Infect Dis J, 25(4):349-53.

Boyanton B, Noroski LM, Reddy H, Dishop MK, Hicks MJ, Versalovic J, Moylett E. (2005). Burkholderia gladioli osteomyelitis in association with chronic granulomatous disease: a case report and review. Ped Infect Dis J., 24:837-839.

Darouiche RO, Berger DH, Khardori N, Robertson CS, Wall MJ, Metzler MH, Shah S, Mansouri MD, Cerra-Stewart C, Versalovic J, Reardon MJ, and Raad II. (2005). Comparison of antimicrobial impregnation with tunneling of long-term central venous catheters: a randomized controlled trial. Ann Surg, 242(2):193-200.

Kaplan SL, Hulten KG, Gonzalez BE, Hammerman W, Lamberth L, Versalovic J, and Mason EO. (2005) Three-year surveillance of community-acquired Staphylococcus aureus infections in children. Clin Inf Dis. 40:1785-1791

Healy M, Huong J, Bittner T, Lising M, Frye S, Raza S, Schrook R, Manry J, Renwick A, Nieto R, Woods C, Versalovic J, and Lupski J. (2005) Microbial DNA typing by automated rep-PCR. J Clin Microb, 43:199-207.

Versalovic J, and Lupski JR. (2002) Molecular detection and genotyping of pathogens: more accurate and rapid answers. Trends Infect Dis 10:S15-S21.

Simko JP, Caliendo AM, Hogle K, Versalovic J. (2002) Differences in laboratory findings for cerebrospinal fluid specimens obtained from patients with meningitis or encephalitis due to herpes simplex virus (HSV) documented by detection of HSV DNA. Clinical Infectious Disease 35:414-419.

Versalovic J and Lupski JR. (1997) Missense mutations in the 3' end of the Escherichia coli dnaG gene do not abolish primase activity but do confer the chromosome segregation-defective ( par ) phenotype. Microbiology 143: 585-594.

Koeuth T, Versalovic J, and Lupski JR. (1995) Differential subsequence conservation of interspersed repetitive Streptococcus pneumoniae BOX elements in diverse bacteria. Genome Research 5:408-418.

Harvey BS, Koeuth T, Versalovic J, Woods CR, and Lupski JR. (1995). Vertical transmission of Citrobacter diversus documented by DNA fingerprinting. Infect Control Epidemiol 16:564-569.

Versalovic J and Lupski JR. (1995). DNA fingerprinting of Neisseria strains by rep-PCR. Meth Mol Cell Biol, 5:96-104.

Versalovic J, Kapur V, Koeuth T, Mazurek, GH, Whittam TS, Musser JM, and Lupski JR. (1995) DNA fingerprinting of pathogenic bacteria by fluorophore-enhanced repetitive sequence-based polymerase chain reaction. Arch Pathol Lab Med, 119:23-29.

Georghiou P, Hamill RJ, Wright CE, Versalovic J, Koeuth T, Watson DA, and Lupski JR. (1995). Molecular epidemiology of infections due to Enterobacter aerogenes: Identification of hospital outbreak strains by molecular techniques. Clin Infect Dis, 20:84-94.

Metzger R, Brown DP, Grealish P, Staver MJ, Versalovic J, Lupski JR, and Katz L. (1994). Characterization of the macromolecular synthesis (MMS) operon from Listeria monocytogenes. Gene , 151:161-166.

Versalovic J, Schneider M, de Bruijn FJ, and Lupski JR. (1994). Genomic fingerprinting of bacteria using repetitive sequence based PCR (rep-PCR). Meth Mol Cell Biol, 5:25-40.

Versalovic J and Lupski JR. (1993). The Haemophilus influenzae dnaG sequence and conserved bacterial primase motifs. Gene, 136:281-286.

Versalovic J, Woods CR, Georghiou PR, Hamill RJ, and Lupski JR. (1993). DNA-based identification and epidemiologic typing of bacterial pathogens. Arch Pathol Lab Med. 117:1088-1098.

Woods C, Versalovic J, Koeuth T, and Lupski JR. (1993). Whole cell rep-PCR allows rapid assessment of clonal relationships of bacterial isolates. J Clin Microbiol, 31:1927-1931.

Versalovic J, Koeuth T, Britton R, Geszvain K, and Lupski JR. (1993). Conservation and evolution of the rpsU-dnaG-rpoD macromolecular synthesis (MMS) operon in bacteria. Mol Microbiol, 8:343-355.

Versalovic J, Kapur V, Mason Jr, EO, Shah U, Koeuth T, Lupski JR, and Musser JM. (1993). Penicillin resistant Streptococcus pneumoniae strains recovered in Houston, Texas: Identification and molecular characterization of multiple clones. J Infect Dis, 167:850-856.

Woods CR, Versalovic J, Koeuth T, and Lupski JR. (1992). Analysis of relationships among isolates of Citrobacter diversus using DNA fingerprints generated by repetitive sequence-based primers in the polymerase chain reaction (rep-PCR). J Clin Microbiol, 30:2921-2929.

Versalovic J, Koeuth T, Zhang YH, McCabe ERB, and Lupski JR,. (1992). Quality control for bacterial inhibition assays: DNA fingerprinting of microorganisms by rep-PCR. Screening , 1:175-183.

Subramanian PS, Versalovic J, McCabe ERB, and Lupski JR. (1992). Rapid mapping of E. coli : Tn 5 insertion mutations by REP-Tn 5 PCR. PCR Methods and Applications, 1:187-192.

Versalovic J, Koeuth T, and Lupski JR. (1991). Distribution of repetitive DNA sequences in eubacteria and application to fingerprinting of bacterial genomes. Nucleic Acids Research , 19:6823-6831.

Versalovic J, Koeuth T, McCabe ERB, and Lupski JR. (1991). Use of the polymerase chain reaction for physical mapping of Escherichia coli genes. J Bacteriol, 173:5253-5255.

Grompe M, Versalovic J, Koeuth T, and Lupski JR. (1990). Mutations in the Escherichia coli dnaG gene suggest coupling between DNA replication and chromosome partitioning. J Bacteriol,173:1268-1278.


Alyamani EJ, Brandt P, Pena JA, Major AM, Fox JG, Suerbaum S, Versalovic J. (2007). Helicobacter hepaticus catalase shares surface-predicted epitopes with mammalian catalases. Microbiology, 153: 1006-1016.

Alyamani E and Versalovic J. (2003). Rapid point of care testing for Helicobacter pylori infection. POC, 2:33-38.

Versalovic J. (2003). Helicobacter pylori: pathology and diagnostic strategies. Amer J Clin Pathol, 119:403-412.

Tuazon OM, Barraquio WL, DeUngria CA, Versalovic J. (2003). Detection of the presence of two Helicobacter pylori clarithromycin resistance markers in a single gastric biopsy by reverse hybridization using a line probe assay and semi-nested allele-specific PCR. Asia Life Sci, 12(1):19-26.

Peña JA, McNeil K, Fox JG, Versalovic J. (2002) Molecular evidence of Helicobacter cinaedi organisms in human gastric biopsies. J Clin Microbiol, 40:1511-1513.

Peña JA, Fox JG, Ferraro MJ, Versalovic J. (2001). Molecular resistance testing of Helicobacter pylori in gastric biopsies. Arch Pathol Lab Med, 125:493-497.

Kwon DH, Peña JA, Osato MS, Fox JG, Graham DY, Versalovic J. (2000). Frameshift mutations in rdxA and metronidazole resistance in North American Helicobacter pylori isolates. J Antimicrob Chemother, 46:793-796.

Patterson MM, Schrenzel MD, Yan Feng Y, Xu S, Dewhirst FE, Paster BJ, Thibodeau SA, Versalovic J, and Fox JG. (2000) Helicobacter aurati sp. nov. cultured from the gastrointestinal tissues of Syrian hamsters. J Clin Microbiol, 38:3722-3728.

Versalovic J, Osato MS, Spakovsky K, Pina Dore M, Reddy R, Stone GG, Shortridge D, Flamm RK, Tanaka SK, Graham DY. (1997). Mutations in the 23S rRNA gene of H. pylori : Point mutations in the 23S rRNA gene associated with different levels of clarithromycin resistance. J Antimicrob Chemother, 40:283-286.

Stone GG, Shortridge D, Versalovic J, Beyer J, Flamm RK, Graham DY, Ghoneim AT, and Tanaka SK. (1997). A PCR oligonucleotide ligation assay to determine the prevalence of 23S rRNA gene mutations in clarithromycin-resistant Helicobacter pylori. Antimicrob Agents Chemother, 41:712-714.

Shortridge D, Stone GG, Flamm RK, Beyer J, Versalovic J, Graham DY, and Tanaka SK. (1997). Molecular typing of Helicobacter pylori isolates from a multicenter U.S. clinical trial by ureC restriction fragment length polymorphism. J Clin Microbiol, 35:471-473.

Stone GG, Shortridge D, Flamm RK, Versalovic J, Beyer J, Idler K, Zulawinski L, and Tanaka, SK. (1996). Identification of a 23S rRNA mutation in clarithromycin-resistant Helicobacter pylori. Helicobacter, 1:227-228.

Versalovic J, Shortridge D, Kibler K, Griffy MV, Beyer J, Flamm RK, Tanaka SK, Graham, DY, and Go MF. (1996). Mutations in 23S ribosomal RNA are associated with clarithromycin resistance in Helicobacter pylori. Antimicrobial Agents Chemother, 40:477-480.

Go MF, Chan KY, Versalovic J, Koeuth, T, Graham, DY, and Lupski JR. (1995). Cluster analysis of Helicobacter pylori genomic DNA fingerprints suggests gastroduodenal disease-specific associations. Scand J Gastroenterol, 30:640-646.


Petrosino JF, Highlander S, Luna RA, Gibbs RA, Versalovic J. Metagenomic pyrosequencing and microbial identification. Clin Chem.; 55:856-866.

Becnel Boyd L, Maynard MJ, Morgan-Linnell SK, Horton LB, Sucgang R, Hamill RJ, Jimenez JR, Versalovic J, Steffen D, Zechiedrich L.(2008) Relationships among ciprofloxacin, gatifloxacin, levofloxacin, and norfloxacin MICs in fluoroquinolone-resistant Escherichia coli clinical isolates. Antimicrob Agents Chemother. (in press)

Zhang M and Versalovic J. (2002). HIV update: diagnostic tests and markers of disease progression and response to therapy. American Journal of Clinical Pathology, 118(Suppl 1):S26-S32.

Versalovic J, Nash Z, Carinhas R, Musher DM, and Baughn RE. (1990) Immunoglobulin class and subclass restriction of autoimmune responses in secondary syphilis. Clinic Exp Immunol 80 :381-386.

Runyan RB, Versalovic J and Shur BD. (1988) Functionally distinct laminin receptors mediate cell adhesion and spreading: The requirement for surface galactosyltransferase in cell spreading. J Cell Biol 107 :1863-1871.

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