Simona Pedrotti, Ph.D.
Started in the lab: March 2011
Alternative splicing of pre-mRNAs greatly expands the coding potential and the complexity of the genome by producing multiple protein isoforms. Regulation of alternative splicing is achieved by the interaction of trans-acting splicing factors with cis-acting sequences found within introns and/or exons. The RNA-binding protein Rbfox1 belongs to the Rbfox family of splicing regulatory proteins which also includes Rbfox2 and Rbfox3. Members of this family regulate the inclusion or exclusion of alternative exons by binding to the sequence (U)GCAUG downstream or upstream of the regulated exon, respectively. Rbfox1 is specifically expressed in the brain, heart and skeletal muscle. Recently, conditional knock out mice for this RNA binding protein in the central nervous system showed a critical role played in neuronal function. The high expression of Rbfox1 in skeletal muscle has challanged us to investigate its role in muscle physiology. I am currently working on the generation of two conditional knock out mouse models for Rbfox1 in the skeletal muscle to determine the roles of Rbfox1 in skeletal muscle development and maintenance.
Pedrotti, S. and Cooper, T.A. (2014) (Mis)-splicing in disease. Journal of Pathology. (In press)
Schmid, R., Grellscheid, S.N., Ehrmann, I., Dalgliesh, C., Danilenko, M., Paronetto, M.P., Pedrotti, S., Grellscheid, D., Dixon, R.J., Sette, C., Eperon, I.C., Elliot, D.J. (2013) The splicing landscape is globally reprogrammed during male meiosis. Nucleic Acids Res. Dec; 41(22):10170-84.
Pedrotti S, Busà R, Compagnucci C, Sette C. (2012) The RNA recognition motif protein RBM11 is a novel tissue-specific splicing regulator. Nucleic Acids Res. Feb;40(3):1021-32
Di Florio A, Adesso L, Pedrotti S, Capurso G, Pilozzi E, Corbo V, Scarpa A, Geremia R, Delle Fave G, Sette C. (2011) Src kinase activity coordinates cell adhesion and spreading with activation of mammalian target of rapamycin in pancreatic endocrine tumour cells. Endocr Relat Cancer Aug 30;18(5):541-54.
Pedrotti S, Sette C. (2010) Spinal muscular atrophy: a new player joins the battle for SMN2 exon 7 splicing. Cell Cycle. Oct 1;9(19):3874-9.
Valacca C, Bonomi S, Buratti E, Pedrotti S, Baralle FE, Sette C, Ghigna C, Biamonti G. (2010) Sam68 regulates EMT through alternative splicing-activated nonsense-mediated mRNA decay of the SF2/ASF proto-oncogene. J Cell Biol. Oct 4;191(1):87-99
Messina V, Di Sauro A, Pedrotti S, Adesso L, Latina A, Geremia R, Rossi P, Sette C. (2010) Differential contribution of the MTOR and MNK pathways to the regulation of mRNA translation in meiotic and postmeiotic mouse male germ cells. Biol Reprod. Oct;83(4):607-15.
Pedrotti S, Bielli P, Paronetto MP, Ciccosanti F, Fimia GM, Stamm S, Manley JL, Sette C. (2010) The splicing regulator Sam68 binds to a novel exonic splicing silencer and functions in SMN2 alternative splicing in spinal muscular atrophy. EMBO J. Apr 7;29(7):1235-47.
Paronetto MP, Cappellari M, Busà R, Pedrotti S, Vitali R, Comstock C, Hyslop T, Knudsen KE, Sette C. (2010) Alternative splicing of the cyclin D1 proto-oncogene is regulated by the RNA-binding protein Sam68. Cancer Res. Jan 1;70(1):229-39.