Amy Brinegar, Ph.D.
Ph.D. Student, Molecular and Cellular Biology
Started in the lab: July 2012
Skeletal muscles undergo vast changes in composition during postnatal development. During this time, a global switch in alternative splicing takes place from the embryonic patterning to the adult patterning. In heart muscle, RNA-binding proteins, particularly CELF1, regulate the developmental switch in alternative splicing patterning. A component of the pathogenesis of myotonic dystrophy type I involves the up regulation of CELF1 protein. Up regulation of CELF1 in skeletal muscle using tetracycline-inducible transgenic mice result in features that resemble myotonic dystriophy including muscle weakness and muscle wasting. CELF1 is likely to function as a global alternative splicing regulator in skeletal muscle that is important for muscle development and regeneration. However, CELF1 and CELF family proteins' function during normal development of muscle is not known. My project will focus on CELF1 and CELF2 proteins role in muscle development during the postnatal stage.
Beilstein MA, Brinegar AE, Shippen DE. Evolution of the Arabidopsis telomerase RNA. Front Genet. 2012;3:188. Epub 2012 Sep 24.