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Path-Immuno - Tom Cooper Lab

Houston, Texas

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Path-Immuno - Tom Cooper Lab
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Reagent Requests

To request reagents from the Cooper lab, please e-mail:Tom Cooper
Include a FedEx number.
Click on plasmid name to obtain its sequence.

Eukaryotic expression plasmids in pcDNA3.1 (all Xpress tagged and ampicillin resistant):

  • tgcugbp (CUG-BP, pcDNA3.1HisC, Xpress tagged)
  • tgetr3 (ETR-3, pcDNA3.1HisC, Xpress tagged)
  • 3.1CBP3R (CELF3, pcDNA3.1HisC, Xpress tagged)
  • NCELF4h (CELF4, pcDNA3.1HisC, Xpress tagged)
  • tgCELF5.4 (CELF5, pcDNA3.1HisC, Xpress tagged)
  • 3.1CELF6orf1 (CELF6, pcDNA3.1HisC, Xpress tagged)
  • PTBΔ294W (PTB dominant negative, pcDNA3.1HisC, Xpress tagged)
  • DBPB (YB-1, pcDNA3.1HisC, Xpress tagged)

Minigenes (splicing reporters, all are ampicillin resistant)

  • RTB33.51 (chicken cTNT)
  • RTB300 (human cTNT)
  • R300TA (human cTNT, CELF non-responsive)
  • RTBPSRAX (ß-globin substitution)
  • 4.11.12muC (test exon sequences, ß-globin context)
  • RG6 (fluorescent protein splicing reporter)
  • FRE5 (cloning vector for fluorescent protein splicing reporter)
  • RHCglo [our favorite splicing minigene reporter; see BioTechniques 41, 177 (2006)]

CTG repeat expression plasmids

  • DT960 (DMPK minigene with 950 repeats in exon 15) amp resistance
  • DMPKS (DMPK minigene with no repeats) kanamycin resistance

In vitro splicing templates (amp resistant)

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