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Neurology

Houston, Texas

The Cullen Building at Baylor College of Medicine.
Department of Neurology
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Isamu Aiba, Ph.D.

Epilepsy Research Fellow (Year 1)

Graduate School

Ph.D., University of New Mexico, N.M.
M.S., Hiroshima University, Hiroshima, Japan

Undergraduate School

B.S., Hiroshima University, Hiroshima, Japan

Research Interests

Experimental expertise

Electrophysiology and fluorescence imaging

  • Brain slice preparation
  • Whole-cell, intracellular sharp electrode recordings
  • Fluorescence imaging (epi-fluorescence, 2-photon microscopy)
  • Craniotomy, extracellular recording in mouse cortex
  • Stereotaxic surgery and virus injection

Biochemistry and molecular biology

  • HPLC
  • Cell culture (plasmid transfection, gene transduction using adeno- and lentivirus)
  • cDNA cloning and generation of recombinant protein
  • Molecular analysis (RT-PCR, northern blotting, western blotting, Gel-shift assays)

Contact Information

Isamu Aiba, Ph.D.
Department of Neurology
Baylor College of Medicine
One Baylor Plaza, MS NB302
Houston, Texas 77030

Email:

Journal Publications (Selected out of 10)

  • Aiba I, West AK, Sheline CT, Shuttleworth CW. Intracellular dialysis disrupts Zn2+ dynamics and enables selective detection of Zn2+ influx in brain slice preparations. J Neurochem. 2013;125(6):822-31. [View journal article]
  • Aiba I, Carlson AP, Sheline CT, Shuttleworth CW. Synaptic release and extracellular actions of Zn2+ limit propagation of spreading depression and related events in vitro and in vivo. J Neurophysiol. 2012;107(3):1032-41. [View journal article]
  • Aiba I, Shuttleworth CW. Sustained NMDA receptor activation by spreading depolarizations can initiate excitotoxic injury in metabolically compromised neurons. J Physiol. 2012;590(Pt 22):5877-93. [View journal article]
  • Durnin L, Dai Y, Aiba I, Shuttleworth CW, Yamboliev IA, Mutafova-Yambolieva VN. Release, neuronal effects and removal of extracellular beta-nicotinamide adenine dinucleotide (beta-NAD(+)) in the rat brain. Eur J Neurosci. 2012;35(3):423-35. [View journal article]
  • Tsai WB, Aiba I, Long Y, Lin HK, Feun L, Savaraj N, et al. Activation of Ras/PI3K/ERK pathway induces c-Myc stabilization to upregulate argininosuccinate synthetase, leading to arginine deiminase resistance in melanoma cells. Cancer Res. 2012;72(10):2622-33. [View journal article]
  • Carter RE, Aiba I, Dietz RM, Sheline CT, Shuttleworth CW. Spreading depression and related events are significant sources of neuronal Zn2+ release and accumulation. J Cereb Blood Flow Metab. 2011;31(4):1073-84. [View journal article]
  • Tsai WB, Aiba I, Lee SY, Feun L, Savaraj N, Kuo MT. Resistance to arginine deiminase treatment in melanoma cells is associated with induced argininosuccinate synthetase expression involving c-Myc/HIF-1alpha/Sp4. Mol Cancer Ther. 2009;8(12):3223-33. [View journal article]
  • Aiba I, Hossain A, Kuo MT. Elevated GSH level increases cadmium resistance through down-regulation of Sp1-dependent expression of the cadmium transporter ZIP8. Mol Pharmacol. 2008;74(3):823-33. [View journal article]
  • Song IS, Chen HH, Aiba I, Hossain A, Liang ZD, Klomp LW, et al. Transcription factor Sp1 plays an important role in the regulation of copper homeostasis in mammalian cells. Mol Pharmacol. 2008;74(3):705-13. [View journal article]
  • Aiba I, Yamasaki T, Shinki T, Izumi S, Yamamoto K, Yamada S, et al. Characterization of rat and human CYP2J enzymes as Vitamin D 25-hydroxylases. Steroids. 2006;71(10):849-56. [View journal article]

Poster and Platform Presentations

  • Aiba I, Shuttleworth CW. Intracellular dialysis can be exploited to distinguish sources of intracellular Zn2+ accumulation in hippocampal neurons. Presented at the 2011 International Society for Zinc Biology (ISZB), Meeting in Melbourne, Australia (Jan. 15-19, 2012).
  • Aiba I, Shuttleworth CW. NMDA receptor activation prolongs the duration of ionic dyshomeostasis following spreading depolarization in murine brain slices. Program No. 780.01. 2011 Neuroscience Meeting Planner. Washington, DC: Society for Neuroscience, 2011. Online.
  • Carter RE, Aiba I, Carlson AP, Sheline CT, Shuttleworth CW. Propagation of spreading depolarizations is limited by the synaptic release and extracellular actions of Zn2+. Program No. 450.05. 2011 Neuroscience Meeting Planner. Washington, DC: Society for Neuroscience, 2011. Online.
  • Aiba I, Shuttleworth CW. NMDA-R activation contributes to metabolic burden following spreading depolarization in murine hippocampal slices. Presented at the Gordon Research Conferences, Excitatory Synapses & Brain Function Meeting in Easton, Mass. (June 26 - July 1, 2011).
  • Aiba I, Shuttleworth CW. Metabolic dependence of zinc effects on spreading depression in murine brain slices. Program No. 872.18. 2010 Neuroscience Meeting Planner. Washington, DC: Society for Neuroscience, 2010. Online.
  • Aiba I, Dietz RM, Sheline CT, Shuttleworth CW. Different roles of extracellular and intracellular Zn2+ in spreading depression-like events in hippocampal slice. Presented at the American Society for Neurochemistry (ASN), 41st Annual Meeting in Santa Fe, N.M. (March 6-10, 2010).

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