Chenghang (Chuck) Zong, Ph.D.
Assistant Professor of Molecular and Human Genetics
Ph.D., University of California, San Diego, 2007
Postdoctoral fellow, University of Illinois at Urbana-Champaign, 2009
Postdoctoral fellow, Harvard University, 2013
The research of our laboratory lies in the interface between novel single cell technologies and quantitative biology. We pursue the development of new quantitative and high-throughput methods for characterizing genomic, epigenetic and transcriptional variations at single cell resolution.
With the rapid development of next-generation sequencing technology, high-throughput sequencing has become a powerful tool for biological research. In our lab, we are interested in examining the genome at single cell resolution, in contrast to the genome averaged from an ensemble of cells. As the demonstration of the principle, we are able to detect the genomic variations between individual cancer cells. We are interested in detecting early events that drive tumorigenesis as well as the early stage of tumor heterogeneity that will influence later tumor development. The lab will focus on pancreatic cancer in particular.
In addition to the genome, we are also interested in developing novel methods for single cell transcriptional and epigenetic profiling. The goal is to capture the development in action, particularly adult stem cell differentiation. Much finer temporal and spatial resolution will allow us to unveil the early signature transcriptome and epigenome changes, which are often buried among downstream responses by late stage and ensemble profiling. The modeling of complex cellular decision-making processes at multiple layers of pathways and regulatory networks will also be pursued in the lab with the enriched heterogeneous and dynamic information extracted from single cells.
For translational research, we will actively pursue clinical applications of single cell techniques, including prenatal genetic testing as well as early cancer diagnosis.
- Zong C, Lu S, Chapman AR, Xie XS (2012). Genome-wide detection of single-nucleotide and copy-number variations of a single human cell. Science 338(6114): 1622-6. PubMed PMID: 23258894
- Lu S, Zong C, Fan W, Yang M, Li J, Chapman AR, Zhu P, Hu X, Xu L, Yan L, Bai F, Qiao J, Tang F, Li R, Xie XS (2012). Probing meiotic recombination and aneuploidy of single sperm cells by whole-genome sequencing. Science 338(6114): 1627-30. PubMed PMID: 23258895
- So LH, Ghosh A, Zong C, Sepúlveda LA, Segev R, Golding I (2011). General properties of transcriptional time series in Escherichia coli. Nat. Genet. 43(6): 554-60. PubMed PMID: 21532574
- Zong C, So LH, Sepúlveda LA, Skinner SO, Golding I (2010). Lysogen stability is determined by the frequency of activity bursts from the fate-determining gene. Mol. Syst. Biol. 2010 6: 440. PubMed PMID: 21119634
- Zeng L, Skinner SO, Zong C, Sippy J, Feiss M, Golding I (2010). Decision making at a subcellular level determines the outcome of bacteriophage infection. Cell 141(4): 682-91. PubMed PMID: 20478257
Chenghang (Chuck) Zong, Ph.D.
Department of Molecular and Human Genetics
Baylor College of Medicine
One Baylor Plaza, MS BCM225
Houston, TX, 77030, U.S.A.