H. David Shine, Ph.D.
Department of Neuroscience
Ph.D.: University of Texas Medical Branch
Postdoctoral training: Harvard Medical School
Gene Therapy for Spinal Cord Injury
The research interests of our laboratory focus on the molecular and cellular bases of disease and trauma of the nervous system with a translational emphasis. Our primary research emphasis is the study of the role of neurotrophic factors in CNS degeneration and regeneration as a consequence of trauma or neurodegenerative disease and their possible therapeutic use. Using viral vectors carrying genes for neurotrophic factors we have shown in animal models of CNS injury we can spare neurons from axotomy-induced neuronal death and support axonal sprouting after neural injury. Recently, we learned that neuroinflammation is required for regenerative effects of the neurotrophic factors and are now studying this phenomenon to determine its mechanism. Techniques used in the laboratory span from gene cloning, through small animal neurosurgery, light and confocal microscopy, neuroimmunology, to animal behavior.
Photomicrograph of a cross section of a rat lumbar spinal cord demonstrating neurotrophic factor-induced axonal plasticity.
Baylor College of Medicine
One Baylor Plaza, Alkek N1130.01
Houston, TX 77030
- Grider MH, Park D, Spencer D, Shine HD. (2009). Lipid raft targeted Akt promotes axonal branching and growth cone expansion via mTOR and Rac1, respectively. J. Neurosci. Res. 87:3033-3042.
- Ayala GE, Dia H, Powel M, Li R, Ding Y, Wheeler TM, Shine HD, Kadmon D, Miles BJ, Ittmann MM, Rowley D. (2008). Cancer related axonogenesis and neurogenesis in prostate cancer. Clinical Cancer Res.; 14:7593-7603.
- Qi H, Li DQ, Shine HD, Chen Z, Yoon KC, Jones DB, Pflugfelder SC. (2008). Nerve growth factor and its receptor TrkA serve as potential markers for human corneal epithelial progenitor cells. Exp. Eye Res. 86:34-40.
- Chen Q, Smith GM, Shine HD. (2007). Immune activation is required for NT-3-induced axonal plasticity in chronic spinal cord injury. Exp. Neurol. 209:497-509.
- Chen Q, Zhou L, Shine HD. (2006). Expression of neurotrophin-3 promotes axonal plasticity in the acute but not chronic injured spinal cord. J. Neurotrauma 23:1254-1260.
- Grider MH, Shine HD. (2006). Selective detection and automated quantification of labeled axons in the CNS. J Neurosci Methods 155:172-179.
- Grider MH, Mamounas LA, Le W, Shine HD. (2005). In situ expression of brain-derived neurotrophic factor or neurotrophin-3 promotes sprouting of cortical serotonergic axons following a neurotoxic lesion. J. Neurosci. Res. 82:404-12.
- Zhou, L and Shine HD. (2003). Neurotrophic factors expressed in the cortex and spinal cord induce axonal plasticity in the injured spinal cord. J Neurosci Res 74:221-226.
- Zhou L, Baumgartner BJ, Hill-Felberg SJ, McGowen, LR and Shine HD (2003) Neurotrophin-3 expressed in situ induces axonal plasticity in the adult injured spinal cord. J. Neurosci. 23: 1424-1431.
- Castro RF, Jackson KA, Goodell MA, Robertson CS, Liu H, and Shine HD. (2002). Failure of Bone Marrow Cells to Transdifferentiate into Neuronal Cells In Vivo. Science, 297: 1299.