AVB Postdoctoral Program: Representative Example of Postdoctoral Training in Biochemistry/Biophysics
Stable Isotope Studies of Apoprotein Synthesis and Assembly (apoB, apo[a], apoE, etc.)
Preceptor: Dr. Farook Jahoor, Joel Morrisett, or Henry Pownall
One of the most powerful technologies available for studying lipoprotein synthesis and catabolism in vivo involves the use of stable isotopes. Trainees in this area attend the weekly seminar series in the AVB Program and weekly journal club meetings in the Children's Nutrition Research Center. Trainees are tutored in isolation of small quantities of apoproteins and lipoproteins from plasma samples using ultracentrifugation, immunoprecipitation and electrophoresis. Fellows then are instructed in the use of high-performance liquid chromatography, capillary gas chromatography-mass spectrometry, and high precision gas isotope ratio mass spectrometry to analyze samples for stable isotope content. Finally, postdoctoral trainees are taught kinetic analysis of stable isotopic tracer data using compartmental models to estimate the rates of synthesis and secretion of apoproteins and lipoproteins. Trainees in this area are encouraged to audit Mathematics and Modeling 409 (Baylor) and Instrumental Analysis (Rice).