Richard Sucgang, Ph.D.
Department of Biochemistry & Molecular Biology
Cell-Based Assay and Screening Service
- B.S. Biology (Genetics) 1989 University of the Philippines at Los Baños, Laguna, Philippines
- Ph.D. - Anatomy and Cell Biology - 1996 - College of Physicians & Surgeons, Columbia University, New York, NY
- Postdoctoral training - Kuspa Lab - Baylor College of Medicine, Houston, TX
High throughput analysis of genomic experiments
Experiments interrogating genomewide effects are now routine thanks to the lowered costs afforded by automation, robotics and updated sequencing technologies. Yet economies of scale do not apply to the management and analysis of these datasets, which remain the major bottleneck in such high throughput biological experiments. Our team collaborates with others to address these computational and biological issues, to bring in high throughput experimental design, ensuring that data produced is trackable and thus amenable to computer assisted analysis. The team developed analysis pipelines for the large scale mutagenesis and functional genomics of dictyostelium, as well as the sequencing, assembly, and annotation of additional dictyostelid species. We continue to develop and maintain the information technology infrastructure to support the workflow of the Cell Based Assay Screening Service, which makes available services such as automated microscopy, robotics, and a large array of shRNA reagents to the BCM community for high throughput screening and experiments. In collaboration with the lab of Dr. Lynn Zechiedrich, we are using next generation sequencing technologies to measure and detect genomic fingerprints that mark the evolution of antibiotic resistance in clinical environments.