Dan Liu, Ph.D.
Department of Biochemistry and Molecular Biology
Cell Based Assay Screening Service (C-BASS) Core Facility
Genetic screens have been a powerful approach for defining signaling and developmental pathways in model organisms. However, such genetic strategies in mammalian systems have been hampered by a lack of tools. The emergence of RNAi as a tool for suppressing gene expression has invigorated the idea that such unbiased approaches can be applied to questions of human biology and disease. With the objective of facilitating BCM investigators in utilizing RNAi technologies, the Cell-based Assay Screening Service core provides instrumentation, resources, assistance, and advice for (1) performing whole-genome or sub-genome scale RNAi screens, (2) utilizing individual lentivirus-based shRNA vectors, (3) large-scale automated manipulation and preparation of shRNA libraries, (4) automated mammalian cell transfection and lentivirus production, (5) high-throughput cell-based assays using automated microscopy or flow cytometry, and (6) data analysis, storage, and management. To ensure optimal use of our services, we provide consultations and training for screen design, assay development, and data acquisition and analysis.
For details, please visit the Cell Based Assay Screening Service website.