Director: Michael M. Ittmann, M.D., Ph.D.
Lab Manager: Patricia Castro, Ph.D.
Co-Director: William Fisher, M.D. (Baylor St. Luke's Medical Center)
Co-Director: Daniel Rosen, M.D.(MEDVA Hospital)
Co-Director: Neda Zarrin-Khameh, M.D., M.P.H. (Ben Taub Hospital)

Services

The Human Tissue Acquisition and Pathology (HTAP) shared resource offers comprehensive pathology support with the following services:

  • Histology and Pathology Services
  • Immunohistochemistry (IHC) and TUNEL Assay
  • Laser Capture
  • Image Analysis
  • Pathology Consultation
  • Tissue Microarray Development
  • Tissue Acquisition and Distribution
  • See full list of services and pricing in iLab

HTAP uses iLabs for all services. For instructions on how to create an account and log-in, see the iLabs instruction sheet.

Histology and Pathology Services

  • Tissue/cell processing and embedding
  • Unstained tissue array sectioning
  • H&E staining
  • Special stains including: PAS, ORO, Trichrome, VVG for elastin, Giemsa
  • Sectioning of thick tissue curls for RNA/DNA isolations

Immunohistochemistry (IHC)

  • IHC using core or researcher provided primary antibodies
  • Double antibody IHC
  • BrdU staining
  • Apoptosis/TUNEL assay

Laser Capture

Laser capture microdissection with the Arcturus XT instruments is a way for researchers to isolate a particular type of cell or a group of cells with very little contamination. This allows DNA and RNA to be isolated from the cells. The service or amplification of the RNA and/or DNA after isolation is also offered. Tissue samples can be submitted as frozen or paraffin sections.

Image Analysis

HTAP can provide state-of-the-art image analysis of standard sections or tissue microarrays using inForm Software. Sections can be stained with standard stains, IHC stains, or immunofluorescence stains. inForm can be trained to find virtually any tissue type, structure or tissue subtype. Moreover, inForm can provide area object counts or be used to automatically assess IHC staining levels on a cell-by-cell and sub-cellular basis, providing extremely accurate and sensitive quantitation.

  • Digital imaging of stained slides
  • Digital scoring of IHC and TUNEL slides
  • Image analysis using Inform and Vectra

Pathology Consultation

One of our experienced pathologists will schedule time to review your slides with you.

Archive Tissue Microarray (TMA)

To request Tissue Microarray Services, send an email to Daniel G. Rosen, M.D.

HTAP manages an archive that contains formalin fixed paraffin-embedded tissue specimens from a variety of organ/disease types. Approximately 10 to 12 years of clinical, pathological and follow-up data is available for most of these tissue specimens. TMAs can be produced using the core's archived collection and/or researcher provided tissue.

  • Identify the cases that meet investigator's project need.
  • Investigators can choose from a library of existing TMAs which include normal and cancer tissues that are site specific or from a multi-tissue array.
  • TMA or cell array can be built using specimens provided by the investigator.
  • Alternatively, a TMA can be designed to address specific questions in the investigator's proposed research.
  • Clinical and pathological data that is associated to each specimen on the array is displayed in the design in an excel format.
  • TMA quality control: Tissue/cell type and diagnosis for each specimen on TMA is confirmed by a pathologist.

Tissue Acquisition and Distribution

Supported by the Dan L Duncan Comprehensive Cancer Center, human tissue banking activities have been expanded to include development of a uniform consent and standardized tissue collection process across all tumor sites at the Michael E. DeBakey Veterans Affairs Hospital and Ben Taub Hospital.

  • Project-based tissue collection
  • Tissue acquisition in support of clinical trials and research protocols
  • Inventory and data management

Request Tissue

Please include the following with your electronic form submission:

Note: All three of these should be attached as one PDF document and emailed to Michael M. Ittmann, M.D., Ph.D.