skip to content »

Genetic Labs

Houston, Texas

Genetic Laboratory
Medical Genetics Laboratories
not shown on screen

Medical Genetics Test Details

View Custom Req   |    Tests in Custom Req: 0
Search Tests: (Search by disease, test name, gene name, test code, or keyword. Return to test index.)

    Browse:  # A B C D E F G H I J K L M N O P Q R S T U V W X Y Z



BRAF Mutation Analysis - Tumor
Test Details
Test Code: 9001
Test Includes: Sequence Analysis of codon 600 and 464-469 of the BRAF gene.


Special Notes: This test can also be performed on FFPE samples (#9000).

Identification of mutations in codon 600 and 464-469 of the BRAF gene.
BRAF is commonly mutated and thereby activated in various human cancers, including non-Hodgkin lymphoma, colorectal cancer, malignant melanoma, thyroid carcinoma, non-small cell lung carcinoma, and adenocarcinoma of lung. BRAF mutations are also found in precancerous lesions, indicating that they occur early in tumorigenesis. The most common mutation of BRAF is the valine to glutamate mutation at BRAF residue 600 (V600E) induced by the T1799A nucleotide transversion within exon 15 (~80-90%) followed by mutations in codons 464-469 (10%). These mutations render tumor resistance to various anti-cancer therapies.

Technical Limitations:

The sensitivity of detection is approximately 5-10 mutated cells per 100 total cells in the sample (5-10%).

Results of this test must always be interpreted in the context of relevant clinical and pathological data, and should not be used alone for a diagnosis of malignancy.

Useful Links:
- Atlas of Genetics and Cytogenetics in Oncology and Haematology
- COSMIC

Useful Publications:
- The Utility of BRAF Testing in the Management of Papillary Thyroid Cancer
- New Driver Mutations in Non-Small-Cell Lung Cancer
Technical Information
Methodology: Sequence analysis is performed in both forward and reverse directions of codon 600 and 464-469 of the BRAF gene.
Gene Name: BRAF
Protein Name: B-Raf proto-oncogene serine/threonine-protein kinase
Sample & Shipping Information
Test Requisition: Tumor Analysis
Specimen Type: Bone Marrow
Requirements: 2-5 mL Bone Marrow (Min: 1 mL) in a EDTA (purple-top) tube(s).

Transport Temperature: Ship at ambient temperature (18-25C/64-77F).

Unacceptable Conditions: Frozen specimens.

Stability: Ambient: 48 hours; Frozen: Unacceptable
Shipping Conditions: Ship at ambient temperature (18-25C/64-77F) in an insulated container by overnight courier. Specimen should arrive in the laboratory within 48 hrs of collection. Do not heat or freeze.

Specimen Type: Fresh Frozen Tissue
Requirements: 150 mg (0.5-2.0 cm3) Fresh tissue snap frozen at -20C. Store at -20C.
Shipping Conditions: Ship on minimum of 10 lbs. of dry ice in an insulated container by overnight courier.


Specimen Type: Leukemic Blood
Requirements: 5-10 mL Leukemic Blood (Min: 1 mL) in a EDTA (purple-top) tube(s).

Transport Temperature: Ship at ambient temperature (18-25C/64-77F).

Unacceptable Conditions: Frozen specimens.

Stability: Ambient: 48 hours; Frozen: Unacceptable
Shipping Conditions: Ship at ambient temperature (18-25C/64-77F) in an insulated container by overnight courier. Specimen should arrive in the laboratory within 48 hrs of collection. Do not heat or freeze.


Turn Around Time: 5 - 7 days
Billing Information
List Price: *For Insurance or Institutional Prices, please call.
CPT Codes: 81210x1


Return to test index