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Click on the name to download each protocol. You will need Adobe Acrobat to read the files.
Protocol
Updated
File size
Adenovirus infection of primary MECs
5.9.2008
60 KB
Avertin (anesthetic for mice)
9.1.2005
68 KB
ChIP Assay (Chromatin immunoprecipitation assay)
9.1.2005
72 KB
Freeze mammary gland pieces (for later transplantation)
9.1.2005
48 KB
Hematoxylin & Eosin (staining of tissue sections)
9.1.2005
252 KB
Hormone treatment, acute (2 day E+P) plus BrdU injection
9.1.2005
64 KB
Immunostaining tutorial
9.1.2005
5.6 MB
Immunostaining: dilutions and conditions
9.1.2005
92 KB
Immunostaining: Representative images
9.1.2005
2.5 MB
Immunofluorescence: General protocol
9.1.2005
64 KB
Immunohistochemistry: General protocol
9.1.2005
84 KB
Immunohistochemistry: "Milk Method"
9.1.0205
60 KB
Immunoprecipitation Assay (for Western blotting)
9.1.2005
56 KB
Lentiviral Production and Titering 5.5.2008
212 KB
Mammosphere Propagation 4.9.2008
40 KB
Masson Trichrome staining (stain for collagen)
9.1.2005
316 KB
Paraformaldehyde, 4% (tissue fixative)
9.1.2005
48 KB
Primary MEC Isolation Short (mammary epithelial cells)
9.1.2005
100 KB
Primary MEC Isolation Overnight (mammary epithelial cells)
5.9.2008
68 KB
Primary MEC extracts (for Western blots/IP assays)
9.1.2005
52 KB
Progesterone receptor/BrdU double immunofluoresence
9.1.2005
244 KB
Retroviral infection of tissue culture cells
9.1.2005
84 KB
Tumor Propagation/Freezing Protocol
4.9.2009
104 KB
TUNEL assay (in situ detection of apoptotic cells)
9.1.2005
176 KB
Wholemount staining (hematoxylin staining of whole mammary glands)
9.1.2005
368 KB
Wholemount staining II (carmine alum staining of mammary glands)
9.1.2005
304 KB
X-Gal staining of tissue (to detect LacZ expression)
5.9.2008
52 KB

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©2001-2005 Baylor College of Medicine
Jeffrey M. Rosen, Ph.D.
Department of Molecular & Cellular Biology
One Baylor Plaza, DeBakey M638
Houston, TX 77030
Phone: 713-798-6211
E-mail: jrosen@bcm.edu

URL: http://www.bcm.edu/rosenlab/protocols.html
Last modified: April 9, 2009