Services
Protein Sequencing
Instrumentation:
- ABI 477A and 492 Procise cLC
Capabilities:
- Sequencing of protein/peptides (soluble or on PVDF)
- Sub 1 pmole sensitivity
- Sequencing of radiolabeled proteins (identification of phosphorylation, acetylation sites, etc)
Peptide Synthesis
Instrumentation:
- ABI 433A synthesizers
Capabilities:
- 0.1 and 0.25mM scales; RP-HPLC purification available
- Synthesis of peptides on MAP resin
- Modified peptides (phosphorylated, cyclized, acetylated, methylated, biotinylated, fluorescent labeled, formylated)
Mass Spec Analysis
Instrumentation:
- ABI/SCIEX 4000 Q-TRAP LC MS/MS System
- ABI/SCIEX 4700 MALDI TOF/TOF System
Capabilities:
- Mass determinations
- Protein identification by peptide mass fingerprinting
- MS/MS analysis
- Peptide sequencing and phosphorylation site identificaiton
Amino Acid Analysis
Instrumentation:
- ABI 420A Analyzer
Capabilities:
- Soluble and PVDF immobilized proteins
- Picomole sensitivity
Enzymatic Digestion of Proteins for Identification
Instrumentation:
- Pharmacia SMART HPLC system with narrow bore reverse phase column
- Protein sequencers and mass spectrometers (see above)
Capabilities:
- Enzymatic digestion of proteins in gel slices
- Less than 10 pmoles of protein needed
- Protein identification and post-translational modification determination by Edman degradation or mass spectrometery
MHC Tetramer Production top
- Guidelines
- Tetramer order form (Note: fill in and submit it on-line or download the form to your local computer and submit it as an attachment via email. Please download Acrobat Reader if necessary)