Lynn Zechiedrich, Ph.D.
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Lynn Zechiedrich, Ph.D.
Kyle and Josephine Morrow Chair in Molecular Virology and Microbiology
Positions
- Kyle and Josephine Morrow Chair in Molecular Virology and Microbiology
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Molecular Virology and Microbiology
Baylor College of Medicine
Houston, TX USA
- Professor
-
Verna and Marrs McLean Department of Biochemistry & Molecular Pharmacology
Baylor College of Medicine
Houston, Texas United States
- Member
-
Dan L Duncan Comprehensive Cancer Center
Baylor College of Medicine
Houston, Texas United States
- Member
-
Dan L. Duncan Comprehensive Cancer Center
Education
- Postdoctoral Training at University Of California, Berkeley
- 07/1997 - Berkeley, California United States
- Biophysics, Microbiology
- PhD from Vanderbilt University
- 08/1990 - Nashville, Tennessee United States
- Biochemistry
- BS from University Of Arkansas
- 05/1985 - Fayetteville, Arkansas United States
- Zoology, Math, Music
Honors & Awards
- Elected Fellow
- American Association for the Advancement of Science (11/2022)
- Elected Fellow
- National Academy of Inventors (12/2017)
- Barbara and Corbin J. Robertson Jr. Presidential Award for Excellence in Education
- "The Barbara and Corbin J. Robertson, Jr. Presidential Award for Excellence in Education emphasizes Baylor's commitment to education and recognizes the excellence in educational contributions of our faculty. The faculty who are honored with this prestigious award have shaped the lives of students through innovative teaching methods and mentoring to prepare our trainees to adopt the mantle of leadership regardless of the career path they choose. They represent the educational efforts of our entire faculty who excel in achieving the goals of our educational mission." Paul E. Klotman, M.D. President, CEO and Executive Dean, Baylor College of Medicine
- 05/2020
- Woman of Excellence
- The Women of Excellence Award was established in 2018 to honor individuals who have demonstrated extraordinary dedication to issues that affect women at Baylor College of Medicine or in the larger community and who exemplify Baylor values.
- Baylor College of Medicine (05/2018)
- Human Frontier Science Program Research Grant
- Human Frontier Science Program (07/2010 - 06/2014)
- John S. Dunn Foundation Collaborative Research Award
- John S. Dunn Foundation, Rice University and Baylor College of Medicine (11/2011 - 10/2013)
- Baylor Research Advocates for Student Scientists (BRASS) mentor of the year award
- Baylor College of Medicine (03/2013)
- Applied Biosystems SOLiDTM 3 System $10K Genome Award
- 02/2009
- Burroughs Wellcome Fund New Investigator Award in the Toxicological Sciences
- Burroughs Wellcome Fund (07/1998 - 06/2002)
Professional Interests
- DNA supercoiling, DNA topoisomerases, antibiotic resistance, gene therapy
Websites
Selected Publications
- Vayssières M, Marechal N, Yun L, Lopez Duran B, Murugasamy NK, Fogg JM, Zechiedrich L, Nadal M, Lamour V "Structural basis of DNA crossover capture by Escherichia coli DNA gyrase." Science. 2024 Apr 12;384:227-232. Pubmed PMID: 38603484
- Radost Waszkiewicz, Maduni Ranasinghe, Jonathan M Fogg, Daniel J. Catanese, Jr., Maria L Ekiel-Jezewska, Maciej Lisicki, Borries Demeler, Lynn Zechiedrich, Piotr Szymczak "DNA supercoiling-induced shapes alter minicircle hydrodynamic properties." Nucleic Acids Res. 2023; Pubmed PMID: 36711572
- Waszkiewicz R, Ranasinghe M, Fogg JM, Catanese DJ, Ekiel-Jeżewska ML, Lisicki M, Demeler B, Zechiedrich L, Szymczak P "DNA supercoiling-induced shapes alter minicircle hydrodynamic properties." Nucleic Acids Res. 2023 May 8;51(8):4027-4042. Pubmed PMID: 36971110
- Chou A, Sucgang R, Hamill RJ, Zechiedrich L, Trautner BW "Mortality difference from Klebsiella aerogenes vs. Enterobacter cloacae bloodstream infections." Access Microbiol. 2023 Feb 27;5(2):acmi000421. Pubmed PMID: 36919079
- Andrew Chou, Richard Sucgang, Richard J. Hamill, Lynn Zechiedrich, Barbara W. Trautner "Mortality difference from Klebsiella aerogenes vs Enterobacter cloacae bloodstream infections." Access Microbiol. 2023 Feb 27;5(2):acmi000421. Pubmed PMID: 36919079
- Maduni Ranasinghe, Jonathan M Fogg, Daniel J Catanese Jr, Lynn Zechiedrich, Borries Demeler "Suitability of double-stranded DNA as a molecular standard for the validation of analytical ultracentrifugation instruments." Eur Biophys J. 2023 Jul 27;DOI: 10.1007/s00249-023-01671-y Pubmed PMID: 37501021
- Blunck BN, Angelo LS, Henke D, Avadhanula V, Cusick M, Ferlic-Stark L, Zechiedrich L, Gilbert BE, Piedra PA "Adult Memory T Cell Responses to the Respiratory Syncytial Virus Fusion Protein During a Single RSV Season (2018-2019)." Frontiers in Immunology. 2022;13:823652. Pubmed PMID: 35422803
- Jonathan M Fogg, Allison K Judge, Erik Stricker, Hilda L Chan, Lynn Zechiedrich "Supercoiling and looping promote DNA base accessibility and coordination among distant sites.." 2021 Sep;12:5683. Pubmed PMID: 34584096
- Blunck BN, Aideyan L, Ye X, Avadhanula V, Ferlic-Stark L, Zechiedrich L, Gilbert BE, Piedra PA. "A prospective surveillance study on the kinetics of the humoral immune response to the respiratory syncytial virus fusion protein in adults in Houston, Texas.." 2021 Feb;39(8):1248-1256. Pubmed PMID: 33509697
- Blunck BN, Aideyan L, Ye X, Avadhanula V, Ferlic-Stark L, Zechiedrich L, Gilbert BE, Piedra PA "Antibody responses of healthy adults to the p27 peptide of respiratory syncytial virus fusion protein." 2021 Jan 24;40(3):536–543. Pubmed PMID: 34903371
- Fogg, JM and Zechiedrich, L "Beyond the static DNA model of Watson and Crick." 2020;746:3–15.
- Arévalo-Soliz LM, Hardee CL, Fogg JM, Corman NR, Noorbakhsh C, Zechiedrich L. "Improving therapeutic potential of non-viral minimized DNA vectors.." 2020 Nov;6(10):1489-1505. Pubmed PMID: 33953961
- Fogg JM Zechiedrich L "Beyond the static DNA model of Watson and Crick." Contemporary Mathematics—Topology and Geometry of Biopolymers. American Mathematical Society. 2020;746:4–15.
- Zechiedrich L Fogg JM "Biophysics meets gene therapy: How exploring supercoiling-dependent structural changes in DNA led to the development of minivector DNA." J Tech Innov. 2019;20:427-440.
- Zechiedrich L "The future of non-viral delivery: niche player or gene therapy cornerstone?." Cell Gene Therapy Insights. 2019 Feb 4;5((S1)):1–7.
- Conley ZC, Carlson-Banning KM, Carter AG, de la Cova A, Song Y, Zechiedrich L. "Sugar and iron: Toward understanding the antibacterial effect of ciclopirox in Escherichia coli." 2019 Jan 11;1:e0210547. Pubmed PMID: 30633761
- Conley, ZC, Bodine, TJ, Chou, A, and Zechiedrich, L "Wicked: The untold story of ciprofloxacin." PLOS Pathog.. 2018 Mar 1;14(3):e1006805. Pubmed PMID: 29494701
- Bizard AH, Yang X, Débat H, Fogg JM, Zechiedrich L, Strick TR, Garnier F, Nadal M "TopA, the Sulfolobus solfataricus topoisomerase III, is a decatenase." Nucleic Acids Res. 2018 Jan 25;46(2):861-872. Pubmed PMID: 29253195
- Bodine TJ, Evangelista MA, Chang HT, Ayoub CA, Samuel BS, Sucgang R, Zechiedrich L "Escherichia coli DNA ligase B may mitigate damage from oxidative stress." 2017 Jul 11;12:e0180800. Pubmed PMID: 28700629
- Wang Q, Irobalieva RN, Chiu W, Schmid MF, Fogg JM, Zechiedrich L, Pettitt BM "Influence of DNA sequence on the structure of minicircles under torsional stress." 2017 Jul 27;45:7633-7642. Pubmed PMID: 28609782
- Hardee CL, Arévalo-Soliz LM, Hornstein BD, Zechiedrich L "Advances in Non-Viral DNA Vectors for Gene Therapy." 2017 Feb 10;8:pii: E65. Pubmed PMID: 28208635
- Chou A, Roa M, Evangelista MA, Sulit AK, Lagamayo E, Torres BC, Klinzing DC, Daroy ML, Navoa-Ng J, Sucgang R, Zechiedrich L "Emergence of Klebsiella pneumoniae ST273 Carrying blaNDM-7 and ST656 Carrying blaNDM-1 in Manila, Philippines." 2016 Oct;22:585-588. Pubmed PMID: 27032000
- Hornstein BD, Roman D, Arévalo-Soliz LM, Engevik MA, Zechiedrich L "Effects of Circular DNA Length on Transfection Efficiency by Electroporation into HeLa Cells." 2016 Dec 5;11:e0167537. Pubmed PMID: 27918590
- Bates D, Pettitt BM, Buck GR, Zechiedrich L "Importance of disentanglement and entanglement during DNA replication and segregation: Comment on: "Disentangling DNA molecules" by Alexander Vologodskii." 2016 Sep;18:160-164. Pubmed PMID: 27666770
- Koire A, Gilbert BE, Sucgang R, Zechiedrich L "Repurposed Transcriptomic Data Reveal Small Viral RNA Produced by Influenza Virus during Infection in Mice." 2016 Oct 27;11:e0165729. Pubmed PMID: 27788253
- Chou A, Nuila RE, Franco LM, Stager CE, Atmar RL, Zechiedrich L "Prevalence of hypervirulent Klebsiella pneumoniae-associated genes rmpA and magA in two tertiary hospitals in Houston, TX, USA." 2016 Sep;65:1047-8. Pubmed PMID: 27392968
- Pandey N, Nobles CL, Zechiedrich L, Maresso AW, Silberg JJ "Combining random gene fission and rational gene fusion to discover near-infrared fluorescent protein fragments that report on protein-protein interactions." 2015 May 15;4:615-24. Pubmed PMID: 25265085
- Irobalieva RN, Fogg JM, Catanese DJ Jr, Sutthibutpong T, Chen M, Barker AK, Ludtke SJ, Harris SA, Schmid MF, Chiu W, Zechiedrich L "Structural diversity of supercoiled DNA." 2015 Oct 12;6:8440. Pubmed PMID: 26455586
- Carlson-Banning, KM and Zechiedrich, L "Antibiotic classes and mechanisms of resistance—considerations for future antibiotic development.." Encyclopedia of Metagenomics. 2014;
- Carlson-Banning KM, Chou A, Liu Z, Hamill RJ, Song Y, Zechiedrich L "Toward repurposing ciclopirox as an antibiotic against drug-resistant Acinetobacter baumannii, Escherichia coli, and Klebsiella pneumoniae." 2013 Jul 23;8:e69646. Pubmed PMID: 23936064
- Swick MC, Evangelista MA, Bodine TJ, Easton-Marks JR, Barth P, Shah MJ, Bormann Chung CA, Stanley S, McLaughlin SF, Lee CC, Sheth V, Doan Q, Hamill RJ, Steffen D, Becnel LB, Sucgang R, Zechiedrich L "Novel Conserved Genotypes Correspond to Antibiotic Resistance Phenotypes of E. coli Clinical Isolates." 2013 Jun 18;8:e65961. Pubmed PMID: 23824211
- Fogg JM, Randall GL, Pettitt BM, Sumners de WL, Harris SA, Zechiedrich L "Bullied no more: when and how DNA shoves proteins around.." Q. Rev. Biophys.. 2012 Aug;45(3):257-99. Pubmed PMID: 22850561
- Zechiedrich L "Editorial Comment to Genome-wide transcriptome analysis of fluoroquinolone resistance in clinical isolates of Escherichia coli.." Int. J. Urol.. 2012 Apr;19(4):368-9. Pubmed PMID: 22394085
- Singh R, Swick MC, Ledesma KR, Yang Z, Hu M, Zechiedrich L, Tam VH "Temporal interplay between efflux pumps and target mutations in development of antibiotic resistance in Escherichia coli.." Antimicrob. Agents Chemother.. 2012 Apr;56(4):1680-5. Pubmed PMID: 22232279
- Catanese DJ, Fogg JM, Schrock DE, Gilbert BE, Zechiedrich L "Supercoiled Minivector DNA resists shear forces associated with gene therapy delivery.." Gene Ther.. 2012 Jan;19(1):94-100. Pubmed PMID: 21633394
- Zhao N, Fogg JM, Zechiedrich L, Zu Y "Transfection of shRNA-encoding Minivector DNA of a few hundred base pairs to regulate gene expression in lymphoma cells.." Gene Ther.. 2011 Mar;18(3):220-4. Pubmed PMID: 20962872
- Swick MC, Morgan-Linnell SK, Carlson KM, Zechiedrich L "Expression of multidrug efflux pump genes acrAB-tolC, mdfA, and norE in Escherichia coli clinical isolates as a function of fluoroquinolone and multidrug resistance." Antimicrob. Agents Chemother.. 2011 Feb;55(2):921-4. Pubmed PMID: 21098250
- Liu Z, Zechiedrich L, Chan HS "Action at hooked or twisted-hooked DNA juxtapositions rationalizes unlinking preference of type-2 topoisomerases.." J. Mol. Biol.. 2010 Jul 30;400(5):963-82. Pubmed PMID: 20460130
- Zechiedrich L, Osheroff N "Topoisomerase IB-DNA interactions: X marks the spot.." Structure. 2010 Jun 9;18(6):661-3. Pubmed PMID: 20541502
- Liu Z, Zechiedrich L, Chan HS "Local site preference rationalizes disentangling by DNA topoisomerases.." Phys Rev E Stat Nonlin Soft Matter Phys. 2010 Mar;81(3):31902. Pubmed PMID: 20365765
- Randall GL, Zechiedrich L, Pettitt BM "In the absence of writhe, DNA relieves torsional stress with localized, sequence-dependent structural failure to preserve B-form.." Nucleic Acids Res.. 2009 Sep;37(16):5568-77. Pubmed PMID: 19586933
- Liu Z, Deibler RW, Chan HS, Zechiedrich L "The why and how of DNA unlinking.." Nucleic Acids Res.. 2009 Feb;37(3):661-71. Pubmed PMID: 19240147
- Morgan-Linnell SK, Becnel Boyd L, Steffen D, Zechiedrich L "Mechanisms accounting for fluoroquinolone resistance in Escherichia coli clinical isolates.." Antimicrob. Agents Chemother.. 2009 Jan;53(1):235-41. Pubmed PMID: 18838592
- Becnel Boyd L, Maynard MJ, Morgan-Linnell SK, Horton LB, Sucgang R, Hamill RJ, Jimenez JR, Versalovic J, Steffen D, Zechiedrich L "Relationships among ciprofloxacin, gatifloxacin, levofloxacin, and norfloxacin MICs for fluoroquinolone-resistant Escherichia coli clinical isolates.." Antimicrob. Agents Chemother.. 2009 Jan;53(1):229-34. Pubmed PMID: 18838594
- Boyd LB, Atmar RL, Randall GL, Hamill RJ, Steffen D, Zechiedrich L "Increased fluoroquinolone resistance with time in Escherichia coli from >17,000 patients at a large county hospital as a function of culture site, age, sex, and location.." BMC Infect. Dis.. 2008;8:4. Pubmed PMID: 18197977
- Morgan-Linnell SK, Hiasa H, Zechiedrich L, Nitiss JL "Assessing sensitivity to antibacterial topoisomerase II inhibitors.." Curr Protoc Pharmacol. 2007 Dec;Unit3.13. Pubmed PMID: 21948169
- Morgan-Linnell SK, Zechiedrich L "Contributions of the combined effects of topoisomerase mutations toward fluoroquinolone resistance in Escherichia coli.." Antimicrob. Agents Chemother.. 2007 Nov;51(11):4205-8. Pubmed PMID: 17682104
- Deibler RW, Mann JK, Sumners de WL, Zechiedrich L "Hin-mediated DNA knotting and recombining promote replicon dysfunction and mutation.." BMC Mol. Biol.. 2007;8:44. Pubmed PMID: 17531098
- Liu Z, Mann JK, Zechiedrich EL, Chan HS "Topological information embodied in local juxtaposition geometry provides a statistical mechanical basis for unknotting by type-2 DNA topoisomerases.." J. Mol. Biol.. 2006 Aug 11;361(2):268-85. Pubmed PMID: 16842819
- Randall GL, Pettitt BM, Buck GR, Zechiedrich EL "Electrostatics of DNA-DNA juxtapositions: consequences for type II topoisomerase function.." J Phys Condens Matter. 2006 Apr 12;18(14):S173-S185. Pubmed PMID: 19088861
- Fogg JM, Kolmakova N, Rees I, Magonov S, Hansma H, Perona JJ, Zechiedrich EL "Exploring writhe in supercoiled minicircle DNA.." J Phys Condens Matter. 2006 Apr 12;18(14):S145-S159. Pubmed PMID: 19337583
- Liu Z, Zechiedrich EL, Chan HS "Inferring global topology from local juxtaposition geometry: interlinking polymer rings and ramifications for topoisomerase action.." Biophys. J.. 2006 Apr 1;90(7):2344-55. Pubmed PMID: 16537549
- Yang S, Lopez CR, Zechiedrich EL "Quorum sensing and multidrug transporters in Escherichia coli.." Proc. Natl. Acad. Sci. U.S.A.. 2006 Feb 14;103(7):2386-91. Pubmed PMID: 16467145
- Lopez CR, Yang S, Deibler RW, Ray SA, Pennington JM, Digate RJ, Hastings PJ, Rosenberg SM, Zechiedrich EL "A role for topoisomerase III in a recombination pathway alternative to RuvABC.." Mol. Microbiol.. 2005 Oct;58(1):80-101. Pubmed PMID: 16164551
- Gruger T, Nitiss JL, Maxwell A, Zechiedrich EL, Heisig P, Seeber S, Pommier Y, Strumberg D "A mutation in Escherichia coli DNA gyrase conferring quinolone resistance results in sensitivity to drugs targeting eukaryotic topoisomerase II.." Antimicrob. Agents Chemother.. 2004 Dec;48(12):4495-504. Pubmed PMID: 15561817
- Buck GR, Zechiedrich EL "DNA disentangling by type-2 topoisomerases.." J. Mol. Biol.. 2004 Jul 23;340(5):933-9. Pubmed PMID: 15236957
- Yang S, Clayton SR, Zechiedrich EL "Relative contributions of the AcrAB, MdfA and NorE efflux pumps to quinolone resistance in Escherichia coli.." J. Antimicrob. Chemother.. 2003 Mar;51(3):545-56. Pubmed PMID: 12615854
- Rahmati S, Yang S, Davidson AL, Zechiedrich EL "Control of the AcrAB multidrug efflux pump by quorum-sensing regulator SdiA.." Mol. Microbiol.. 2002 Feb;43(3):677-85. Pubmed PMID: 11929524
- Deibler RW, Rahmati S, Zechiedrich EL "Topoisomerase IV, alone, unknots DNA in E. coli.." Genes Dev.. 2001 Mar 15;15(6):748-61. Pubmed PMID: 11274059
- Zechiedrich EL, Khodursky AB, Bachellier S, Schneider R, Chen D, Lilley DM, Cozzarelli NR "Roles of topoisomerases in maintaining steady-state DNA supercoiling in Escherichia coli.." J. Biol. Chem.. 2000 Mar 17;275(11):8103-13. Pubmed PMID: 10713132
- Zechiedrich EL, Crisona NJ "Coating DNA with RecA protein to distinguish DNA path by electron microscopy.." Methods Mol. Biol.. 1999;94:99-107. Pubmed PMID: 12844866
- Zechiedrich EL, Khodursky AB, Cozzarelli NR "Topoisomerase IV, not gyrase, decatenates products of site-specific recombination in Escherichia coli.." Genes Dev.. 1997 Oct 1;11(19):2580-92. Pubmed PMID: 9334322
- Khodursky AB, Zechiedrich EL, Cozzarelli NR "Topoisomerase IV is a target of quinolones in Escherichia coli.." Proc. Natl. Acad. Sci. U.S.A.. 1995 Dec 5;92(25):11801-5. Pubmed PMID: 8524852
- Zechiedrich EL, Cozzarelli NR "Roles of topoisomerase IV and DNA gyrase in DNA unlinking during replication in Escherichia coli.." Genes Dev.. 1995 Nov 15;9(22):2859-69. Pubmed PMID: 7590259
- Crisona NJ, Kanaar R, Gonzalez TN, Zechiedrich EL, Klippel A, Cozzarelli NR "Processive recombination by wild-type gin and an enhancer-independent mutant. Insight into the mechanisms of recombination selectivity and strand exchange.." J. Mol. Biol.. 1994 Oct 28;243(3):437-57. Pubmed PMID: 7966272
- Adams DE, Shekhtman EM, Zechiedrich EL, Schmid MB, Cozzarelli NR "The role of topoisomerase IV in partitioning bacterial replicons and the structure of catenated intermediates in DNA replication.." Cell. 1992 Oct 16;71(2):277-88. Pubmed PMID: 1330320
- Corbett AH, Zechiedrich EL, Osheroff N "A role for the passage helix in the DNA cleavage reaction of eukaryotic topoisomerase II. A two-site model for enzyme-mediated DNA cleavage.." J. Biol. Chem.. 1992 Jan 15;267(2):683-6. Pubmed PMID: 1309770
- Corbett AH, Zechiedrich EL, Lloyd RS, Osheroff N "Inhibition of eukaryotic topoisomerase II by ultraviolet-induced cyclobutane pyrimidine dimers.." J. Biol. Chem.. 1991 Oct 15;266(29):19666-71. Pubmed PMID: 1655791
- Osheroff N, Zechiedrich EL, Gale KC "Catalytic function of DNA topoisomerase II.." Bioessays. 1991 Jun;13(6):269-73. Pubmed PMID: 1654050
- Zechiedrich EL, Osheroff N "Eukaryotic topoisomerases recognize nucleic acid topology by preferentially interacting with DNA crossovers.." EMBO J.. 1990 Dec;9(13):4555-62. Pubmed PMID: 2176156
- Andersen AH, Christiansen K, Zechiedrich EL, Jensen PS, Osheroff N, Westergaard O "Strand specificity of the topoisomerase II mediated double-stranded DNA cleavage reaction.." Biochemistry. 1989 Jul 25;28(15):6237-44. Pubmed PMID: 2551368
- Zechiedrich EL, Christiansen K, Andersen AH, Westergaard O, Osheroff N "Double-stranded DNA cleavage/religation reaction of eukaryotic topoisomerase II: evidence for a nicked DNA intermediate.." Biochemistry. 1989 Jul 25;28(15):6229-36. Pubmed PMID: 2551367
- Osheroff N, Zechiedrich EL "Calcium-promoted DNA cleavage by eukaryotic topoisomerase II: trapping the covalent enzyme-DNA complex in an active form.." Biochemistry. 1987 Jul 14;26(14):4303-9. Pubmed PMID: 2822084
Memberships
- Biophysical Society
- Member (01/2017 - 12/2033)
- American Society For Microbiology
- Member (08/1997)
- American Association for the Advancement of Science
- Member (01/1996 - 01/2012)
- American Association for the Advancement of Science
- Member (12/2017 - 12/2033)
- American Society for Biochemistry and Molecular Biology
- Member (01/1996 - 12/2032)
- American Society for Cell Biology
- Member (01/2007 - 01/2010)
- American Chemical Society
- Member (01/2001 - 01/2010)
- American Mathematical Society
- Member (01/1999 - 12/2033)
- American Society for Microbiology, Texas Branch
- Member (01/1997 - 12/2033)
Funding
- Harnessing Supercoiling to Regulate DNA Activity - #R35 GM141793 (10/01/2021 - 09/30/2026) Grant funding from National Institutes of Health
- DNA Damage Recognition in Linear and Supercoiled DNA - #MCB 2107527 (04/01/2021 - 03/31/2025) Grant funding from National Science Foundation
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