Viral PCR Analysis

The detection of viral genomic nucleic acid sequences by PCR or RT-PCR provides a highly sensitive and specific method of diagnosis of viral infection. These techniques have facilitated the association of different virus types with acute and chronic disease that was either unclear or overlooked using the classical virologic techniques of culture, serology, or immunohistochemistry. The limitations of these techniques were due to various reasons, including but not limited to, sensitivity, the latent or persistent state of the virus, and the immunocompetence of the host. In addition, these techniques often required several weeks for the accumulation and interpretation of the data. PCR is extremely sensitive and rapid, and when associated with other molecular biological methods can facilitate an accurate description of the state of the infecting virus at the molecular level. For additional information on the role of viral infection in myocarditis, as well as our role in the discovery of viral agents as one of the underlying causes of myocarditis, please read the article "How Viral Infections Fit Into Myocarditis" (PDF), which was published in CAP Today in October, 2004.

A variety of tissue and fluid samples can be analyzed for the presence of virus genomic sequences. These include myocardial samples (biopsy, explant, or autopsy), lung, liver, brain, tracheal aspirates, and amniotic fluid. For tissue samples, the method of preservation is important for the ability to amplify genetic material. Please carefully read the specimen handling instructions.