Reagent requests
To request reagents from the Cooper lab, please contact: Tom Cooper
Include a FedEx number for labile reagents and faster delivery.
Click on plasmid name to obtain its sequence
Eukaryotic expression plasmids in pcDNA3.1 (all Xpress tagged and ampicillin resistant):
- tgcugbp (CUG-BP, pcDNA3.1HisC, Xpress tagged)
- tgetr3 (ETR-3, pcDNA3.1HisC, Xpress tagged)
- 3.1CBP3R (CELF3, pcDNA3.1HisC, Xpress tagged)
- NCELF4h (CELF4, pcDNA3.1HisC, Xpress tagged)
- tgCELF5.4 (CELF5, pcDNA3.1HisC, Xpress tagged)
- 3.1CELF6orf1 (CELF6, pcDNA3.1HisC, Xpress tagged)
- PTBΔ294W (PTB dominant negative, pcDNA3.1HisC, Xpress tagged)
- DBPB (YB-1, pcDNA3.1HisC, Xpress tagged)
Minigenes (splicing reporters, all are ampicillin resistant)
- RTB33.51 (chicken cTNT)
- RTB300 (human cTNT)
- R300TA (human cTNT, CELF non-responsive)
- RTBPSRAX (ß-globin substitution)
- 4.11.12muC (test exon sequences, ß-globin context)
- RG6 (fluorescent protein splicing reporter)
- FRE5 (cloning vector for fluorescent protein splicing reporter)
- RHCglo [our favorite splicing minigene reporter; see BioTechniques 41, 177 (2006)] (MTA required)
CTG repeat expression plasmids
- DT960 (DMPK minigene with 950 repeats in exon 15) amp resistance
- DMPKS (DMPK minigene with no repeats) kanamycin resistance
In vitro splicing templates (amp resistant)
- E46NB (chicken cTNT)