Human CELF/BRUNOL Genes
| Cooper1,6 | Good2 Notes | Timchenko/ Swanson3 | % identity Sakaki5 | CUG-BP | Expression | Chrom. |
| CUG-BP | BRUNOL2 | CUG-BP1# | --- | ubiquitous | 11p11 | alt spliced, 2 promoters |
| ETR-3 | BRUNOL3 | CUG-BP2# NAPOR1,2,3* | 78% | brain, emb musc | 10p13 | alt spliced, 5 promoters |
| CELF3 | BRUNOL1 | 44% | brain only | 1q21 | alt spliced | |
| CELF4 | BRUNOL4 | 42% | brain enriched | 18q12 | alt spliced | |
| CELF5 | BRUNOL5 | 39% | brain only | 19p13 | alt spliced | |
| CELF6 | BRUNOL6 | 46% | brain, kidney | 15q24 | alt spliced |
# In early papers, different phosphoisoforms of CUG-BP were called CUG-BP1 (hyperphosphorylated) and CUG-BP2 (hypophosphorylated)4. CUG-BP2 now refers ETR-3/BRUNOL3 in GenBank.
* NAPOR1, NAPOR2, and NAPOR35 have different N-termini due to use of three alternative promoters (see below).
The ETR-3/BRUNOL3/NAPOR gene contains several alternative promoters (first exons) which generate different N-termini
References
1. Ladd, A. N., Charlet-B.N., and T. A. Cooper. 2001. The CELF family of RNA binding proteins is implicated in cell-specific and developmentally regulated alternative splicing. Mol Cell Biol 21:1285-1296.
2. Good, P. J., Q. Chen, S. J. Warner, and D. C. Herring. 2000. A family of human RNA-binding proteins related to the Drosophila Bruno translational regulator. J Biol Chem 275:28583-28592.
3. Timchenko, L. T., J. W. Miller, N. A. Timchenko, D. R. Devore, K. V. Datar, L. J. Lin, R. Roberts, C. T. Caskey, and M. S. Swanson. 1996. Identification of a (CUG)n triplet repeat RNA-binding protein and its expression in myotonic dystrophy. Nucl Acids Res 24:4407-4414.
4. Roberts, R., N.A. Timchenko, J.W. Miller, S. Reddy, C.T. Caskey, M.S. Swanson, and L.T. Timchenko. 1997. Altered phosphorylation and intracellular distribution of a (CUG)n triplet repeat RNA-binding protein in patients with myotonic dystrophy and in myotonin protein kinase knockout mice. Proc. Natl. Acad. Sci. 94:13221-13226.
5. Choi, D. K., T. Ito, F. Tsukahara, M. Hirai, and Y. Sakaki. 1999. Developmentally regulated expression of mNAPOR encoding an apoptosis-induced ELAV-type RNA binding protein. Gene 237:135-142.
6. Ladd, A.N., Nguyen, N.H. Malhotra, K and Cooper, T.A. (2004) CELF6, a member of the CELF family of RNA binding proteins, regulates MSE-dependent alternative splicing. J. Biol. Chem. 279,17756-17764.