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Molecular Virology and Microbiology

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Molecular Virology and Microbiology
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Ramig Lab Research
Annotated Table of the Properties of Rotavirus SA11 Proteins

(updated April 2003)

Genome Segment*
Size (BP)
Gene Product (Protein Function )
Location in Virus Particle
Copy Number/ Particle
Protein Size
Cognate Proteins O=Orbivirus R=Reovirus
GenBank Accession Number(s)
Functions and Properties (Ref)
Amino Acids
Daltons
1 3302 VP1(Pol) Inner Capsid 5-fold Axis 12 1088 12005 O:VP1
R: lambda 3		 X16830 (56)
  • RNA-dependent RNA Polymerase (87)
  • Part if minimal replication complex (63,87)
  • Virus specific 3'-mRNA binding (61,62)
  • Part of viron transcription complex with VP3 (11,73)
2 2690 VP2 (T1) Inner Capsid 120 880 102431 O: VP3
R: lambda 1		 X16831 (56)
  • Inner capsid structural protein (8)
  • Non-speccific ss & dsRNA binding (10)
  • Myristoylated (15)
  • Cleaved (23,86)
  • Part of minimal replication complex (63)
  • Leucine zipper (56)
  • nteracts with VP5 (7)
3 2690 VP3 (Cap) Inner capsid 5-fold axis 12 835 98120 O:VP4
R: lambda 2		 X16062 (44) X16387 (56)
  • Guanylyltransferase (45,68)
  • Methyltransferase (13)
  • Basic protein (44,56)
  • Part of virion transcription complex with VP1 (11,73)
  • Non-specific ssRNA binding (62)
4 2362 VP4 Outer capsid spike 120 776 8678 - D16346 (77) X14204 (55)
  • Dimers form outer capsid spike (3)
  • Virus infectivity enhanced by trpsin cleavage to VP5* & VP8* (22,46)
  • Hemagglutinin (26,38)
  • Cell attachment protein (47,75,85)
  • P-type neutralization antigen (32,58)
  • VP5* permeabilizes membranes (16)
  • Crystal structure of VP8 fragment - galectin fold (19)
  • Traf2 signaling (43)
  • Protection from virulent challenge (33)
VP5* 529 60000
VP8* 247 28000
5 1611 NSP1 Non-structural 0 495 58654 - L18944 (35) X14914 (57)
  • Associates with cytoskeleton (34)
  • Extensive sequence diversity (20,42,57)
  • Two conserved cysteine-rich zinc fingers (57,60)
  • Virus-specific 5'-mRNA binding (24,62)
  • Interacts with host IFN regulatory factor 3 (29)
6 1356 VP6 (T13) Middle Capsid 780 387 44816 O:VP7 L15384 (48) L33365 (48) M27824 (76)
  • Major viron capsid protein (49,72)
  • Middle capsid structural protein (72)
  • Homottrimeric quaternary structure (72)
  • Subgroup antigen (30,39)
  • Myristoylated (15)
  • Protection from virulent challenge (? mechanism)(11,84)
  • Crystal structure (50)
  • Hydrophobic (48,76)
7 1049 NSP3 Non-structural 0 315 34600 - M87502 (51)
  • Homodimer (51,66)
  • Virus-specific 3'-mRNA binding (69,70)
  • Binds host eIF4G1 & circularizes mRNA on initiation complex (67)
  • Involved in translational regulation and host shut-off (14,59,82)
  • Crystal structure: NSP3 N-term fragment with 3'-viral ent with eIF4G1 fragment (31)
8 1059 NSP2 (VIP) Non-structural 0 317 36700 O:NS2
R:sigma NS 		L04531 (64)
  • Non-specific ssRNA binding (41,62)
  • Accumulates in viroplasm (65)
  • Involved in viroplasm formation with NSP5 (25)
  • NTPase activity (79)
  • Helix destabilization activity (78)
  • Functional octamer (79,80)
  • Binds NSP5 and VP1 (1,40)
  • Regulates NSP5 autophosphorylation (1)
  • Crystal structure: HIT-like fold (37)
9 1062 VP7 Outer capsid glyco- protein 780 326 37368 - K02028 (4)
  • Outer capsid structural glycoprotein (21,49)
  • G-type neutralization antigen (32)
  • N-linked high mannose glycosylation and trimming (21)
  • RER transmembrane protein, cleaved signal sequence (22)
  • Calcium binding (119)
  • Protection from virulent challenge (33)
10 751 NSP4 Non-structural 0 175 20290 AF087678 (9)
  • Enterotoxin (6)
  • Receptor for buding of double-layer particles through ER membrane (5,53)
  • RER transmembrane glycoprotein (22)
  • Calcium/Strontium binding site (36)
  • N-linked high mannose glycosylation (21)
  • Protection from virulent challenge (24)
  • Host cell intracellular calcium mobilization (81)
11 667 NSP5 Non-strucutral 0 198 21725 - X07831 (54) M28347 (83)
  • Product of second out-of-frame ORF (52)
  • Interacts with NSP5 (27)
  • Localizes to viroplasm (52)
NSP6 Non-structural 0 92 11012 -

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