pJT106:  Human Notch 2 ICN domain with COOH terminal Flag epitope tag PCR cloned 
into T/A vector T-easy (Promega).  The open reading frame for Notch 2 ICN is 
from bases 2414 to 72 (antisense).

GGGCGAATTGGGCCCGACGTCGCATGCTCCCGGCCGCCATGGCGGCCGCGGGAATTCGATTCGATGGATCCTTACTTGTC
ATCGTCGTCCTTGTAGTCCGCATAAACCTGCATGTTGTTGTGTGGTGGCTCAGACATGTGTGTCCCAGGTCCCCGCTGAC
CTCCTCCAGCACCCCCAGGGGTAGGGCTGGTGGTCACATCTGACCAGTCAGAAGCAGAGTGGGGTGATGAACTTGACCAC
TGGTCAGGAGACTCTGGGGATGGCGTCAGGTAGGGATGCTCACCCTGGAGGTGACCACTGTGACTGGGTGTTCGCTCAGC
AGCATTTGAGGAAGCATAACTGTGCTGTGAAGGGGGTGTGGGGTACTTGCCCACAGAGGCTGGGAAAGGATGATAGGCTG
GGAGAATGGTCTGAGCTACCTGCCCGTCCTGCTGGGGCATCATGGCAGTGGGGAAAGCCACACTGGGCAAACGGGCCATT
TCTGGAATCTGGTACATGGTGGGCAGGGGGCCCGCAACAGCTGGAGGGCAGGTGGACTGAGGCTGGGGAGCCCCCGCTGG
TTGGGCAATACTGCCTTTAGGGATGAGCTGGAAAGTCACAATGGGGGGCAAGGGCTCCCGAGGGGTGGTTATGTGCTTCC
CTTCAGGTGGCCTGCTCTGGGGAGCTATGCCAGGATGGGTGCCCTCAGCTGGAGCCAGGACCATACCAAACATCTCATTG
TACTGGGTCTCATTCACCTCCATGCGGTTCATCCAATCTGCTGGGACTGGGACTGGATGGAGCCTACTCAAGCTTCCAGC
ACTGCCACTGCCTGGAGACACAATGTGGTGGTGGGATAGCAACTGGCTCACTGAGGGAAGCACAGTGCTGGCCCCATGTG
CCAAAGGCTGCATTTCATGAAGGTTAGAAAAAGATAGTGCATGCTGGGCATGGACTGGGGCAGGAGGGGCGGCAGTGGCC
AACATAGGGTTGGGTGAGGCCTGTAAGATCCCAGGGGATGTAATCATTGGAGAGGATGTGGTGTCGGAAACATACGTGTG
AGGAGATTCTAGGGAATCAACAGGGGATAAAGTTACTGAACTCTCAGACAGTTGGACCTTCTCACTCAGAGACTTCTTCC
TCCTACTACCCTTGGCATCCTTTGCCTCCTTGGCAAGGTTAGGGAGGCTAGTAGGCATGGTACTCTTGGCACTGGGCCGT
CTAGACTTCTTGCCCATTGGGGTGTGCTTCAGGCTGAGGAAAGATCTGTTGGGCCCACAGATGACAGGTGAGAGAACAGA
AGTCAACACGGTGCCTGGAGGGCTTGGGGTCACATTGTATTCATCCAGAAGGCGCACAATGTCATGGTGCATGCGATCCC
GAGCCACATCCCGGGGAAGACGATCCATATGGTCTGTGATGTCTCGATTGGCAAAATGGTCTAACAGGATCTTGGCTGCT
TCATAGCTCCCCTCCCGGGCAGCAAGAAACAGAGGTGTCTCTTCCTTGTTGTCCTGCATGTCTCGGTTGGCCCCATTTTT
CAACAACAAAAGAGTTGCCTCCACATTATTGACAGCAGCTGCCCAGTGAAGAGCAGATTTTCCATGGTCATCCACTGCAT
TCACATCCGCTTGGCAGTTGATCAGTTCTGCCACCATTCCCTCCACAGCCAGGCGGGCAGCCAGGATCAGGGGTGTAGTA
CCATCATTCATCCTGGCATCTAGATCAGTTACTCGGTTGCGAATCAGAATCTGGAAGACACCTTGGGCATCAGCTGCCAC
TGCAGCATGGAGTGGACAGCGGCCCATGTTGTCCTGGGCATTGGCATCTGCACCTGCATCCAGGAGACGCTTGGCAGCAT
CAGCCCGTGAGTAGCGGGCTGCAAGGTGCAGGGCCATCTCACCAGTCCGGTCTGTCTGGGCCTGGAGGCTGGCACCCTGG
TAGACCAAGTCTGTGATGATGTTAGCAGAAGAGTCCTCTGCATCTTCATCTTCATCACTCAAATCTGAGCTGCCTCCTCG
GAGAGAAGCCAACATCAATGGGGTGCAGCCATCTGGGCCACGGACATTCACATCTAACACATCCACCTCCTGCTCTGCCT
GAGGAGGGGTGAGAGCCAGCGATGGTGTCCTACGGATGTCTGCAGCTTCAAGGTGCTGCTGTGTCCATGGCCGTCGATCA
ATGGGGTCATCTTCTTCTGAGAGTAAGGCCTCATCTTCAGCCTTTACTTTCTTTGGCTGGGGCCCTTCATCATCGACCCA
GTGTTCACTTGTTCCAGTACCAATTAGGTTAGCTTCTGAGACTTGCACTGAGAGATTTTTCAGCCCCACAGCATCCTGTC
CCACTGGCTCACGACGCTTGTGATTGCTTGCATCTCGGCGAAGAGTGAAACCTTCAGGCAGCCAGAGAGAGCCATGCTTA
CGCTTTCGTTTCATGATGGAATTCATCGAATCACTAGTGAATTCGCGGCCGCCTGCAGGTCGACCATATGGGAGAGCTCC
CAACGCGTTGGATGCATAGCTTGAGTATTCTATAGTGTCACCTAAATAGCTTGGCGTAATCATGGTCATAGCTGTTTCCT
GTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATG
AGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAAT
GAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCG
GTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGG
AAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCC
GCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCG
TTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTC
GGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTG
TGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGAC
TTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTG
GTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAG
TTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGA
AAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGAT
TTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTA
TATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCA
TCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGAT
ACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTC
CTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTG
CGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCA
ACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAA
GTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGC
TTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTC
AATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCT
CAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTC
ACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAAT
ACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTA
TTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGATGCGGTGTGAAATACCGCACAG
ATGCGTAAGGAGAAAATACCGCATCAGGAAATTGTAAGCGTTAATATTTTGTTAAAATTCGCGTTAAATTTTTGTTAAAT
CAGCTCATTTTTTAACCAATAGGCCGAAATCGGCAAAATCCCTTATAAATCAAAAGAATAGACCGAGATAGGGTTGAGTG
TTGTTCCAGTTTGGAACAAGAGTCCACTATTAAAGAACGTGGACTCCAACGTCAAAGGGCGAAAAACCGTCTATCAGGGC
GATGGCCCACTACGTGAACCATCACCCTAATCAAGTTTTTTGGGGTCGAGGTGCCGTAAAGCACTAAATCGGAACCCTAA
AGGGAGCCCCCGATTTAGAGCTTGACGGGGAAAGCCGGCGAACGTGGCGAGAAAGGAAGGGAAGAAAGCGAAAGGAGCGG
GCGCTAGGGCGCTGGCAAGTGTAGCGGTCACGCTGCGCGTAACCACCACACCCGCCGCGCTTAATGCGCCGCTACAGGGC
GCGTCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAAGGGCGATCGGTGCGGGCCTCTTCGCTATTACGCCAGCTGGCG
AAAGGGGGATGTGCTGCAAGGCGATTAAGTTGGGTAACGCCAGGGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAG
TGAATTGTAATACGACTCACTATA