Ling Laboratory

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pPDL176A EBNA2 ORF 1053-2516 in pSG5. EBNA2 ORF flanked by EcoRI and BglII sites

pPDL151 wt EBNA2 cloned as BglII fragment into BglII site of pSG5 (Stratagene). BglII fragment 1055-2565, EBNA2 ORF 1096-2559

pPDL152 mutEBNA2 cloned as BglII fragment into BglII site of pSG5 (Stratagene). BglII fragment 1055-2565, EBNA2 ORF 1096- 2559. Xba I site introduced to change aa 326,327 from WW to SR

pJT105 Human Notch 1 IC cloned into T-easy. Notch1IC ORF 71-2467; Flag 2468-2494. The Notch1 IC ORF-Flag is flanked by EcoRI and BglII sites

pJT106 Human Notch 2 ICN domain with COOH terminal Flag epitope tag PCR cloned into T/A vector T-easy (Promega). The open reading frame for Notch 2 ICN is from bases 2414 to 72 (antisense).

pJT111 Human Notch1IC cDNA with 3’ terminal Flag epitope. EcoRI-BglII fragment cloned into EcoRI-BglII site of pSG5.

pJT112 Human Notch2IC cDNA with 3’ Flag epitope. EcoRI-BamHI fragment cloned into EcoRI-BglII site of pSG5.

pJT125 EBNA-LP cDNA with 2 W repeats. 3’ Flag epitope. Cloned as an EcoRI-BglII fragment into the EcoRI-BglII site in pSG5

pJT125 EBNA-LP cDNA (1060-1587) with 2xW1W2 repeats and COOH-term Flag in pSG5 expression vector

pJT123A 8x head-to-tail copies EBNA2 Cp enhancer (-430 to –330) cloned as BglII/BamHI fragment (bases 42-889) into BglII site of pGL3-promoter vector.

pJT174 human Sp100A cDNA cloned into EcoRI and BglII sites in pSG5 vector. Deleted for aa3-152. HA-epitope tag at C-term.

pAG 115 RhEBNA2 (Macaque LCV) cDNA EcoRI-BglII fragment cloned into EcoRI-BglII sites of pSG5.

pAG131 Lentivirus vector, IRES-eGFP (J. Virol. (2001) 75:5899-5912) eGFP gene 8915-9634, IRES 8333-8914

pAG138 (pLIG.EBNA2), EBNA2 ORF 8294-9757

pAG143 (pLIG.hNotch1IC), hNotch1IC 8294-10717

pAG 155 EBNA2 cDNA with 3' HA epitope. EcoRI-BglII fragment cloned into EcoRI-BglII site of pSG5.

pAG 156 DPPR-EBNA2 cDNA with 3' HA epitope. EcoRI-BglII fragment cloned into EcoRI-BglII site of pSG5.

pAG 190 D3-30EBNA2 cDNA with 3' HA epitope. EcoRI-BglII fragment cloned into EcoRI-BglII site of pSG5

pAG 191 D3-52EBNA2 cDNA with 3' HA epitope. EcoRI-BglII fragment cloned into EcoRI-BglII site of pSG5.

pAG 196 D3-30EBNA2 EcoRI-BglII fragment cloned into EcoRI-BglII site of pSG5. Amino acids 3-30 in the amino-terminus of EBNA2 are deleted

pRSP363 EBNA2 ORF 1053-2525 flanked by EcoRI and BglII sites. EBNA2 sequence is identical to the published sequence from Dambaugh et al PNAS, 1984 81:7632.

pRSP438 EcoRI-BglII fragment cloned into pSG5 (1043-1567). EBNA-LP delCR3 ORF 1049-1561.

pRSP441 pRSP441: Wild-type Human Sp100 A cDNA. cDNA cloned as an EcoRI/BglII fragment into the EcoRI/BglII sites in pSG5.

pRSP462 human Sp100A cDNA cloned into EcoRI and BglII sites in pSG5 vector. Deleted for aa153-286. HA-epitope tag at C-term.

pRSP463 human Sp100A cDNA cloned into EcoRI and BglII sites in pSG5 vector. Deleted for aa287-333. HA-epitope tag at C-term.

pRSP464 human Sp100A cDNA cloned into EcoRI and BglII sites in pSG5 vector. Deleted for aa334-407. HA-epitope tag at C-term.

pRSP470 human Sp100A cDNA cloned into EcoRI and BglII sites in pSG5 vector. Deleted for aa408-480. HA-epitope tag at C-term. SV40 NLS at C-term.

pRSP614 murine PML cDNA with carboxy-terminal HA epitope tag cloned into the XbaI/Not1 sites of the pCI expression vector.