Integrated Microscopy Course
Integrated Microscopy, 2009
This class is offered during the 2nd term (elective, 3 credits)
Class begins Oct. 15 and ends Dec. 10 (tentative)
Class is held every Thursday from 1-5 p.m.
Michael Mancini, Ph.D.
B.R. Brinkley, Ph.D.
Michael Mancini, Ph.D.
B.R. Brinkley, Ph.D.
Leoncio Vergara, M.D.
Sean Hartig, Ph.D.
Ivan Uray, M.D., Ph.D.
Debra Townley, B.S.
Cullen Bldg., Room 130A, every Thursday from 1-2 p.m. (some lectures may run overtime)
Cullen Bldg., Rooms 118A, 120A, 123A and 125A: immediately following lecture, 2-5 p.m. (some labs may run until 6 p.m.)
Oct. 15, 2009
Dr. Mancini - Overview of Course: Lectures, Project, Oral Exam
Dr. Vergara - Fundamentals of Light Microscopy I: Basic optics, light path, lenses.
Dr. Mancini - Basic Light Microscopy: Hands on introduction to the light microscope: Kohler illumination and alignment, brightfield, DIC; overview of the course
Oct. 22, 2009
Dr. Vergara - Fundamentals of Light Microscopy II: Absorption, fluorescence, confocal, deconvolution, multiphoton, spectral microscopy
Drs. Mancini, Vergara, Uray, and Hartig - Intermediate Light Microscopy: Hands on introduction to confocal and deconvolution microscopy; continued training on standard microscope
Oct. 29, 2009
Dr. Mancini/Dr. Vergara - Advanced Light Microscopy: Introduction to High Throughput Microscopy, Live Imaging, FRAP, FRET, TIRF, HTM
Drs. Mancini, Vergara, Hartig - Cell Labeling/Start of Student Projects: Brightfield; fluorescent labeling of coverslips and multi-well plates with several dyes, antibodies, or expressed fluorecent proteins; image acquisition on Axioplan2, confocal, deconvolution and high throughput microscopes
Nov. 5, 2009
Dr. Mancini - Molecular Cytology: Seeing is Believing (or is it?): Antibodies, epitope tags, XFPs, reporters, expermental design
Drs. Uray and Hartig - Continuation of student projects and training on all scopes. Live and HTM demos
Nov. 12, 2009
Dr. Brinkley - Introduction to Electron Optics (Electron Microscopy I) and Specimen Preparation (Electron Microscopy II)
Dr. Brinkley, Debra Townley - Transmission Electron Microscopy: Training on transmission electron microscope
Drs. Uray and Hartig - Continuation of student projects and training on all scopes
Nov. 19, 2009
Dr. Mancini - Automated Microscopy I: overview of recent paradigm shift toward automated microscopy for routine bench science and library screening
Drs. Uray and Hartig - Continuation of student projects and training on all scopes
Dec. 3, 2009
Dr. Mancini - Automated Microscopy II: Image Analyses and Reporting
Drs. Uray and Hartig - Continuation of student projects and training on all scopes
Dec. 10, 2009
Dr. Mancini - Final Exam: oral, flexible date dependent on other exams
Oral Exam (~30 min): Discussion with Dr.Mancini to determine the depth of microscopy acquired during the course
Projects Due: Projects culminate in a powerpoint presentation of images collected from the lab. Essentially, the powerpoint should reflect your accumulated understandings of light and electron microscopy with text and demonstration images from each scope. Example powerpoints from previous classes will be available (see below)