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Available
Arrays Clone
Selection Core
Services Contacts
revised 6-7-01 CMN |
Available Services Description:
For the busy researcher we recomend this service which includes RNA labeling by dye-nucleotide, hybridization, scanning and quantitation. A minimum of 10 ug total RNA or 1 ug polyA RNA is required. In either case, for best results the RNA should be quality controlled through RNA quantitaiton and gel electrophoresis. Description:
We will scan any slide in up to four fluorophores (see scanner specs) and up to 5 micron resolution. The slides must be standard sized microscope slides (either metric 25mm x 75 mm or English 1.0" x 3.0" and 1 mm thick). Data will be provided as uncompressed tagged image file format (TIFF). Optionally, files can be compressed to JPEG or GIF for viewing purposes. Description:
Microarray images are quantitated using GSI Lumonics Quantarray or Nu-Tec Gleams software. Data is provided as a raw flat file. Standard Microarray Core Facility slides will also be provided with Microsoft Excel templates. Templates for custom arrays are available upon request. Description:
Want to learn the basics of using microarrays, then sign up for a training session. These private sessions can cover labeling, hybridization, quantitation and analysis. For more information see our contact list. Description:
Perhaps you have a set of cDNA clones, genomic samples, BAC clones, oligonucleotides or other DNA materials that you would like to print onto microscope slides. The Microarray Core Facility can print up to 6,000 elements in duplicate onto plain microscope slides or a few thousand elements onto membrane-coated microscope slides. The MCF can assist in the design, preparation and analysis of custom microarrays whether for gene expression, genotyping, comparative genomic hybridization and more. Scanning services and custom analysis are also available. Custom arrays may also be design from existing clone sets.
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