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Available
Arrays
Mouse 9K
Mouse BMAP 9K Mouse
BMAP 6K Mouse QC
Human 9K
Human QC
Dictyostelium 8K
Custom Arrays
Clone
Selection
current clone lists
Core
Services
hybridization, scanning,
quantitation, custom arrays
Contacts
who/where we are
revised
6-7-01 CMN
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Available
Microarrays
Mouse
9K $250
Description:
We have obtained from Incyte a set of 8700 sequence-verified
clones from the Mouse GEM 1 set containing cDNA fragments from the
mouse genome. The average insert size is 1500 bp. Presently 2500
clones contain fragments of known genes. This array contains the
entire set printed in duplicate on a single glass microscope slide.
The 9K Array contains genes from the above set as well as 1152 genes
from the BMAP Brain clone set. The array contains multiple controls
including three control targets from Saccharomyces cerevisiae and
9 mouse "housekeeping genes." Control yeast RNA is available.
Download
Gene List Sample Image Sample Data
*Array costs include scanning and image quantitation.
Mouse
BMAP 9K $250
Description:
We have obtained from Incyte a set of 11,000 sequence-verified
clones from the Mouse BMAP set containing cDNA fragments from
many brain-tissue specific tissues including the cerebellum,brain
stems,olfactory bulbs, hypothalamus, prefrontal cortex, amygdala,
basal ganglia, pineal glands, corpus striatum, hippocampus, enthorinal
cortex, dentate gyrus and the spinal cord of C57/BLK6 mice. This
array contains 9,000 of the genes printed in duplicate on a single
glass microscope slide. The array contains multiple controls including
three control targets from Saccharomyces cerevisiae and 9 mouse
"housekeeping genes." Control yeast RNA is available.
Download
Gene List Sample
Image Sample
Data
*Array costs include scanning and image quantitation
Mouse
BMAP 6K $250
Description:
We have obtained from Research Genetics a set of 6,000 sequence-verified
clones from the Mouse BMAP set containing cDNA fragments from
mouse embryo, neonatal, and adult eye tissues. This array
contains 6,000 genes from the set printed in duplicate on a single
glass microscope slide. The array contains multiple controls including
three control targets from Saccharomyces cerevisiae and 9 mouse
"housekeeping genes." Control yeast RNA is available.
Download
Gene List Sample
Image Sample
Data
*Array costs include scanning and image quantitation
Mouse
QC $12.50
Description:
The Mouse QC (Quality Control) Array is a good starter
array for labs beginning array experiments. It constists of a 5x5
array of 25 genes replicated 32 times on the chip. The targets represent
genes that have shown high expression on past arrays and can give
the investigator(s) a good idea if they have successfully labeled
and hybridized their samples to the array.
Download
Gene List Sample
Image Sample
Data
*Array costs include scanning and image quantitation.
Human
9K $250
Description:
We have obtained from Genome Systems a set of 7075 sequence-verified
clones from the Human UniGEM V (now Incyte GEM 1) set containing
cDNA fragments from the human genome. The average insert size is
1000 bp. Presently 4096 clones contain fragments of known genes
and an additional 577 are annotated ESTs. The 9K Array contains
7296 genes from the above set as well as 1536 genes from the Human
cDNA Seqence Verified set. The array contains multiple controls
including three control targets from Saccharomyces cerevisiae and
9 human "housekeeping genes." Control yeast RNA is available.
Download
Gene List Sample
Image Sample
Data
*Array costs include scanning and image quantitation.
Human
QC $12.50
Description:
The Human QC (Quality Control) Array is a good starter
array for labs beginning array experiments. It constists of a 5x5
array of 25 genes replicated 32 times on the chip. The targets represent
genes that have shown high expression on past arrays and can give
the investigator(s) a good idea if they have successfully labeled
and hybridized their samples to the array.
Download
Gene List Sample
Image Sample
Data
*Array costs include scanning and image quantitation.
Dictyostelium
8K Coming Soon $---
Description:
This array represents a collection of 7700 cDNA and genomic
clones of Dictyostelium Discoideum genes. Each target is
arrayed in duplicate on a single glass microscope slide. The set
is partially characterized with ??? known genes and ??? ESTs. The
array contains multiple controls including three "housekeeping
genes" and a ribosomal RNA message target. Ribosomal primer
for reverse transcription is available.
Download Gene List Sample
Image Sample
Data
*Array costs include scanning and image quantitation.
Custom
Arrays from $20
Description:
Perhaps you have a set of cDNA clones, genomic samples,
BAC clones, oligonucleotides or other DNA materials that you would
like to print onto microscope slides. The Microarray Core Facility
can print up to 6,000 elements in duplicate onto plain microscope
slides or a few thousand elements onto membrane-coated microscope
slides. The MCF can assist in the design, preparation and analysis
of custom microarrays whether for gene expression, genotyping, comparative
genomic hybridization and more. Scanning services and custom analysis
are also available. Custom arrays may also be design from existing
clone sets. We recommend purified
DNA with a concentration of at least 50 ng/uL or 50 uM for oligonucleotides.
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