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Thrombosis Research Section Faculty
Faculty (Secondary Appointment)
Research Interests:
We are studying the mechanisms of neutrophil transendothelial migration.
This work includes analysis of several families of adhesion molecules
in the cascade leading to leukocyte emigration. Of particular focus
are members of the CD18 (ß2) integrins, intercellular adhesion molecules
(e.g., ICAM-1, CD54), selectins (e.g., E-selectins, CD62E), junctional
adhesion molecules (JAM-A, JAM-B and JAM-C), and CD31. Animal models
of inflammation (e.g., intravital microscopy and myocardial ischemia)
are studied in mice with targeted deletion or mutations of the specific
adhesion molecules. Models in vitro include analysis of how fluid
shear stress modulates adhesion and emigration, especially regarding
site selection and kinetics of transmigration.
Selected Publications:
1. A. Chakraborty, E.R. Hentzen, S.M. Seo, and C.W. Smith. Granulocyte
colony-stimulating factor promotes adhesion of neutrophils. Am.J.Physiol.Cell
Physiol. 2003;284:C103-C110.
2. E. Hentzen, D. McDonough, L. McIntire, C.W. Smith, H.L. Goldsmith,
and S.I. Simon.Hydrodynamic shear and tethering through E-selectin
signals phosphorylation of p38 MAP kinase and adhesion of human neutrophils.
Annals Biomed. Engineer 2003;30:987-1001.
3. M.M. Mariscalco, W. Vergara, J. Mei, E. Smith, and C.W. Smith.
Mechanisms of decreased leukocyte localization in the developing host.
Am.J.Physiol.Heart Circ.Physiol. 2002;282:H636-H644.
4. S.M. Seo, L.V. McIntire, and C.W. Smith. Effects of IL-8, Gro-a,
and LTB4 on the adhesive kinetics of LFA-1 and Mac-1 on human neutrophils.
Am.J.Physiol.Cell.Physiol. 2001;281:C1568-C1578.
5. P.K. Gopalan, A.R. Burns, S.I. Simon, S. Sparks, L.V. McIntire,
and C.W. Smith. Preferential sites for stationary adhesion of neutrophils
to cytokine-stimulated HUVEC under flow conditions. J.Leuk.Biol.
2000;68:47-57.
6. S.I. Simon, Y. Hu, D. Vestweber, and C.W. Smith. Neutrophil tethering
on E-Selectin activates b2-integrin binding to ICAM-1 through a MAP
kinase signal transduction pathway. J.Immunol. 2000;164:4348-4358.
7. A.R. Burns, R.A. Bowden, S.D. MacDonell, D.C. Walker, T.O. Odebunmi,
E.M. Donnachie, S.I. Simon, M.L. Entman, and C.W. Smith. Analysis
of tight junctions during neutrophil transendothelial migration. J.Cell
Sci. 2000;113:45-57.
8. A.R. Burns, R.A. Bowden, Y. Abe, D.C. Walker, S.I. Simon, M.L.
Entman, and C.W. Smith. P-Selectin mediates neutrophil adhesion to
endothelial cell borders. J.Leuk.Biol. 2000;65:299-306.
9. H. Lu, C.W. Smith, J. Perrard, D. Bullard, L.P. Tang, S.B. Shappell,
M.L. Entman, A.L. Beaudet, and C.M. Ballantyne. LFA-1 is sufficient
in mediating neutrophil emigration in Mac-1-deficient mice. J.Clin.Invest.
1997;99:1340-1350.
10. Y. Abe, C. Ballantyne, and C.W. Smith. Functions of Domain 1
and 4 of vascular cell adhesion molecule-1 in a4 integrin-dependent
adhesion under static and flow conditions are differentially regulated.
J.Immunol. 1996;157:5061-5069.
11. J.K. Mickelson, N.M. Lakkis, G. Villarreal-Levy, B.J. Hughes,
and C.W. Smith. Leukocyte activation with platelet adhesion after
coronary angioplasty, a mechanism for recurrent disease? J.Am.Coll.Card.
1996;28:345-353.
 ©1995-2003
Baylor College of Medicine
Email: medicine@bcm.tmc.edu
URL: http://public.bcm.tmc.edu/medicine/cwsmith.htm (Modified: June 4, 2003)
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