Alternative Amplification Methods for Expression Arrays

Alternative Amplification Methods For Expression Arrays

The expression array companies do not necessarily endorse the following kits. These possibilities are to be considered if the required starting material specified in the specific array’s protocol cannot be attained.

NuGen

For Affymetrix GeneChip Arrays:

Ovation System V2:
System requires 5-100ng of intact total RNA in 5uL

WT-Ovation Pico System:
System requires 500pg-50ng of intact total RNA in 5uL

WT-Ovation FFPE System:
System requires 50-100ng of total RNA in 5uL

For Spotted Arrays (Agilent or NimbleGen):

Ovation Aminoallyl System:
System requires 5-100ng of total RNA in 5uL

Ambion MessageAmp:
For Eukaryotic Affymetrix GeneChip Arrays:

MessageAmp II-Biotin-Enhanced Single Round Amplification Kit:
System requires 50ng of intact total RNA (max is 5,000ng, recommended is 1,000ng) in 10uL.

MessageAmpII-Biotin-Enhanced Two Rounds of Amplification Kit: MessageAmpII & MessageAmp II-Biotin-Enhanced Single Round Amplification Kit:
System requires 100pg-50ng intact total RNA

For Bacterial Affymetrix GeneChip Arrays:

MessageAmp-II-Bacteria RNA Amplification Kit:
System requires 100ng-1000ng intact total RNA

For Spotted Arrays (Agilent or NimbleGen):

Amino Allyl MessageAmp II:
System requires 100pg-100ng intact total RNA

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